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Aluminum toxicity in alfalfa seedlings: Effects on root growth and antioxidative defense in three cultivars
(World Alfalfa Congress, 2025-11-03) Stepić, Marija; Joković, N.; Vitorović, J.; Bekčić, Filip; Petrović, Mirjana; Andjelković, Snežana; Radović, Jasmina
Aluminum toxicity is a major limiting factor for alfalfa (Medicago sativa L.) growth in acidic soils (pH<5.5), posing a significant challenge to global agriculture. High concentrations of toxic aluminum ions induce oxidative stress, leading to reduced yield in alfalfa growing regions in the whole world. Toxic effects are manifesting primarily in the root after which affect the development of overall plant tissues. Particularly susceptible are alfalfa seedlings. This study aimed to assess the effect of aluminum toxicity on root growth and biochemistry in seedlings of three alfalfa cultivars (Zuzana, K-28, and O-66). The perfored platform containing seeds was placed put in half-strength Hoagland’s nutrient solution (pH 6) for 7 days. After that period, the pH of solution was adjusted to 4.5 and aluminium chloride was added to a final concentration of 100 μM. The response of three alfalfa cultivars to aluminum stress was evaluated after 14 days. The most affected traits were root elongation and lateral root formation in all cultivars, both of which showed the significant decrease in aluminum treatment. The inhibited activity of two antioxidative enzymes, superoxide dismutase (SOD) and ascorbate peroxidase (APX), also occurred in all cultivars. Root length was significantly reduced in all cultivars compared to the control groups, among which O-66 showed the most pronounced reduction. In contrast, cultivars Zuzana and K-28 showed no significant difference from each other but displayed significantly longer roots than cultivar O-66 under aluminum stress. Lateral root formation followed a similar trend, further highlighting the toxic impact of aluminum on root development in all three cultivars. The activity of APX and SOD was significantly reduced in all three cultivars under aluminum treatment. The greatest percentage inhibition of both enzymes was observed in cultivar O-66, indicating a higher sensitivity to aluminum-induced oxidative stress compared to the other two cultivars. Aluminum toxicity significantly decreased root growth and antioxidative enzyme activity in alfalfa seedlings, with cultivar O-66 showing the highest sensitivity. Root elongation and lateral root formation were most reduced in O-66, along with the greatest inhibition of SOD and APX activity. In contrast, Zuzana and K-28 exhibited greater tolerance. These findings highlight the impact of aluminum stress on alfalfa and point to interpopulation variability among these three cultivars.
An initial metabolomic study on Hungarian clover
(Palacký University Olomouc, 2023-09-10) Petrović, Mirjana; Zornic, Vladimir; Lugić, Zoran; Stepić, Marija; Prijović, Mladen; Vymyslicky, Tomas; Andjelković, B
Hungarian clover (Trifolium pannonicum Jack.) is the youngest member of the genus Trifolium and is classified as a minorspeciesin terms of cultivation areas. It has a long lifespan, up to 15 years (Nechaeva et al., 2020), is resistant to freezing, diseases, and drought, has good antioxidant potential, and produces one stable harvest annually. Despite these advantages, however, it is not used enough in the production of fodder.
An overview of the flora of the special nature reserve "Osredak" a decade upon the completion of the conservation study
(Centre for Evaluation in Education and Science (CEON/CEES), 2022-01-25) Petrović, Mirjana; Prijović, Mladen; Živković-Antić, Ivana; Stojanović, Verica; Vymyslický, Tomaš; Stepić, Marija; Babić, Snežana; Sokolović, Dejan
Since 2020, the "Osredak" Special Nature Reserve has been a protected natural area of the Republic of Serbia, of regional, i.e. great importance. It is situated in central Serbia, along both banks of the West Morava. The area was designated as protected in order to conserve the bog habitats of the Morava River valley, as well as old bayous, ponds and lakes, which were created by decades of gravel extraction. The public-utility company "Kruševac" manages this area, and at its request, cooperation was established with the Istitute for fodder crops Kruševac in order to update the Reserve's flora inventory, with an emphasis on protected plant species, but also on invasive alien species. A list of all plant taxa that have been recorded in the Reserve so far (169 species) and specific solutions that might contribute to the conservation and improvement of natural values of the "Osredak" special nature reserve have also been included in this paper.
ANTIFUNGAL ACTIVITY OF RHIZOBIA AGAINST RED CLOVER PATHOGENS FROM GENUS FUSARIUM
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia University of Belgrade, Faculty of Agriculture, Serbia Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB) Italy, 2023-10-05) Bekčić, Filip; Duduk, Natasa; Lugić, Zoran; Radović, Jasmina; Babić, Snežana; Stepić, Marija; Andjelković, Snežana
Red clover (Trifolium pratense L.) is an important forage legume that can be found in nature or as cultivated crop in monoculture or in grass-legume mixtures. The great importance of red clover is the fact that it lives in symbiosis with Rhizobium leguminosarum bv. trifoli. In addition to promoting plant growth, productivity and biomass, the bacteria also have an important role in disease control. Apart from useful microorganisms, many soil pathogenic microorganisms inhabit red clover roots and under favorable conditions they cause infections and diseases of the cultivated plants. On the infected red clover plants Fusarium spp. can cause several symptoms: vascular wilt on older plants, dumping of seedlings, root rot, rot of root base and crown rot. In the present study, the antagonistic potential was evaluated for two bacteria isolates preliminarily determined as R. leguminosarum bv. trifoli (CD1 and CD6) against three isolates Fusarium spp. (G1, G2 and G3) who were obtained from the roots of symptomatic red clover plants. The research was performed in in vitro agar plate (PDA medium) by methods of dual culture (“bacterial ring”). The comparison of growth assessment of fungi after they were grown together with rhizobia in a single plate and growth of fungi in individual plates was performed. Both rhizobia isolates showed significant antifungal activity against all the tested phytopathogenic fungi. Growth inhibition of G1, G2 and G3 was evaluated (%) using both rhizobia isolates, CD1 varied from 30 to 43 % and CD6 from 14 to 32 %.
Antimicrobial potential of the methanol and water extract of Potentilla argentea L. aerial parts and roots
(Prirodno-matematički fakultet, Univerzitet u Banjoj Luci, 2025-11-13) Duboja Krstić, A.; Novaković, M.; Pešić, S.; Stojanović, D.; Stepić, Marija; Joković, N.; Vitorović, J.
Potentilla argentea L., is traditionally used in Kruševac, Serbia, as an herbal decoction for the treatment of gastrointestinal disorders. This study evaluated and compared the antimicrobial activity of both the plant root and aerial parts (stems, leaves and flowers). P. argentea samples were collected during two different periods: March and May. Samples were dried, ground, and subjected to ultrasonic extraction using methanol (ME) and water (AE). The aqueous extract of the root was prepared following the traditional method of tea preparation, with modifications involving 24-hour steeping in water prior to filtration, rather than immediate use as in typical preparation. Antimicrobial activity was assessed by determining Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) against seven American Type Culture Collection (ATCC) bacterial strains: Staphylococcus aureus ATCC 6538, Bacillus cereus ATCC 11778, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Salmonella enteritidis ATCC 13076, Enterobacter aerogenes ATCC 13048 and Pseudomonas aeruginosa ATCC 9027. None of the extracts inhibited E.coli, while the strongest antimicrobial activity was observed againts S. enteritidis (0,0098/0,0098 mg/ml). ME from aerial parts exhibited stronger antimicrobial activity compared to root extracts. However, root ME exhibited greater antimicrobial activity against B. cereus compared to aerial part ME, while it showed no antimicrobial activity against E. aerogenes and P. aerogenes. No significant difference was observed between root and aerial parts ME against S. aureus and E. faecalis, though samples harvested in May exhibited greater antimicrobial acitivity in both cases. AE from roots collected in March showed no antimicrobial activity.
Biomass quality of the faba bean lines
(Maize Research Institute Zemun Polje, 2025-09-29) Milenković, Jasmina; Zornic, Vladimir; Andjelković, Snežana; Bekčić, Filip; Stepić, Marija; Petrović, Mirjana
Many studies have shown that various parts of the faba bean plant (Vicia faba L.) contain useful compounds, including protein, fiber, and a wide range of bioactive substances. The chemical composition of faba bean plant sections varies depending on the plant's genotype and growing conditions. Aside from the grain, the faba bean plant contains relatively little protein. This study evaluated ten promising faba bean lines with high grain protein content identified in prior research. These lines are derived from local Serbian faba bean populations, and previous research indicated that their grain has high protein content (more than 27%). A two-year experiment with two replications was conducted in the experimental field of the Institute for Forage Crops Kruševac, utilizing a random block design. The plants were cut at the green pod stage when the seeds had formed but the plants remained green because higher protein content and higher biomass quality were expected. The following parameters were assessed using the Weende method: crude protein, crude cellulose, ash, crude fat, and nitrogen-free extract (NFE). The results showed significant variance across the studied faba bean lines. Dry mass content ranged from 90% to 91.4%, crude protein content from 18.3% to23.3%, crude cellulose from 14.2% to 25.6%, crude ash from 6.3% to 9.4%, crude fat from 0.4% to 2.1%, and NFE content from 41.2% to 50.3%. As expected, the protein level in the biomass was high at this stage of mowing, possibly due to the developed grains. This biomass is a high-quality feed for fresh and ensiling applications. Furthermore, the high levels of some of the other assessed parameters indicate that more research is needed on the dry matter content of the entire faba bean plant at this cutting stage.
GENETIČKI ODGOVOR LUCERKE NA TOKSIČNO DELOVANJE JONA ALUMINIJUMA NA KISELOJ HIDROPONSKOJ KULTURI
(Društvo genetičara Srbije, Beograd, 2023-10-16) Stepić, Marija; Bekčić, Filip; Prijović, Mladen; Radović, Jasmina; Zdravković, Jasmina
Toksičan efekat aluminijuma veoma je važan faktor koji ograničava rast i razviće korena lucerke (Medicago sativa L.) na kiselim zemljištima što dovodi do smanjenja prinosa. Cilj eksperimenta bio je da se utvrdi uticaj aluminijuma na: dužinu korena, svežu nadzemnu masu, svežu masu korena, broj bočnih korenova, dužinu bočnih korenova i na rastojanje do prvog bočnog grananja na korenu kod klijanaca tri sorte lucerke (Zuzana, K-28, O-66) u hidroponskoj kulturi u kontrolisanim uslovima. Korišćen je Hoaglandov rastvor ½ jačine pH vrednosti 4.5, za tretman je korišćen aluminijum u formi aluminijum-hlorida (koncentracije 100 µM) i za analize je korišćeno 40 klijanaca po sorti.
Morpho-biochemical response perennial ryegrass (Lolium perenne L.) populations to water shortage
(Palacký University Olomouc, 2023-09-10) Prijović, Mladen; Sokolović, Dejan; Babić, Snežana; Petrović, Mirjana; Stepić, Marija; Lazarević, Đorđe; Sabovljević, Aneta
Drought is one of the most significant effects of global climate change. It is expected to be more intense in some parts of southern Europe and the Balkan Peninsula in the next decade. Drought stress is the most important restriction factor for food and feed productivity. Perennial ryegrass (Lolium perenne L.) forms the basis of grassland production in temperate pastures and represents one of the most important forage grasses globally.
Nutritive value of field pea - oat and common vetch - oat bicrops
(National Library of Serbia, 2023) Marković, Jordan; Milenković, Jasmina; Petrović, Mirjana; Zornic, Vladimir; Racić, Nedeljko; Stepić, Marija; Blagojević
Peas and vetches are the most important plant species for the production of forages, where they can be used as hay, haylage or silage. Due to their specific characteristics - tendency to lodging and high buffering capacity, they are usually grown in a mixture with small grains as support crops to prevent or reduce lodging, reduce buffering capacity, and at the same time obtain biomass suitable for the ensiling process. In pure crops, peas are most often used as green feeds or hay at the stage of first pod formation, while the best quality of vetch dry matter has been achieved by using it at the stage of full flowering or the beginning of first pod formation, when up to 25% of crude protein is obtained in the biomass of pure culture. The nutritive value of pure crops of peas, common vetch and oats, and their mixtures is presented as a function of the structure of the mixtures and the stage of use. Special attention is paid to the content of cell wall components and the dry matter digestibility of these feeds. Because of all of these mentioned above, it is particularly important to choose the right harvesting time to balance the yield and nutritional value of feeds used in bicrops.
NUTRITIVE VALUE OF ITALIAN RYEGRASS-RED CLOVER MIXTURES DEPENDING ON THE SEEDING RATE IN THE MIXTURES
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia University of Belgrade, Faculty of Agriculture, Serbia Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB) Italy, 2023-10-05) Marković, Jordan; Vasic, Tanja; Zornic, Vladimir; Racić, Nedeljko; Bekčić, Filip; Stepić, Marija; Kostić, Ivica
More profitable production and better forage quality is achieved by growing grasses and legumes in mixtures. Growing grasses in a mixture with legumes influences a significant amount of nitrogen fixed by Rhizobium bacteria and reduce the using of nitrogen mineral fertilization. Italian ryegrass is an ideal species for growing in a mixture with red clover. The aim of this study was to determine the nutritive value of Italian ryegrass – red clover mixtures depending on the seeding rate of these plants in the mixtures, in the fist and the second cut. Since the trial was not fertilized with N the legumes were required to supply N. The experiment was established at the experimental field of the Institute for forage crops Kruševac, Serbia, according to a total randomized block system in three replications. The results of this study showed that better nutritive value was determined in the mixtures with higher seeding rate of red clover in the mixture. In the first cut, the nutritive value of the mixture with the highest Italian ryegrass seeding rate was similar with nutritive value of red clover monocrop. Generally, in conclusion, growing Italian ryegrass in a bi-crop with red clover produces forage of high nutritive value suitable for ruminants feeding.
Penicillium crustosum - проузроковач плаве трулежи плодова нектарине
(Društvo za zaštitu bilja Srbije, 2022-11-28) Bekčić, Filip; Vico, I.; Vučković, N.; Mikić, S.; Stepić, Marija; Andjelković, Snežana; Duduk, Natasa
PREDSELEKCIONO ISPITIVANJE GENOTIPOVA MAĐARSKE DETELINE
(Društvo genetičara Srbije, Beograd, 2023-10-16) Petrović, Mirjana; Zornic, Vladimir; Milenković, Jasmina; Stepić, Marija; Radović, Jasmina; Tomić, Dalibor; Andjelković, Snežana
Vrste roda Trifolium ostvaruju simbiotsku vezu sa zemljišnim bakterijama, pripadnicima vrste Rhizobium leguminosarum bv. trifoli koja se ogleda u formiranju sitnih kvržica koje su raspoređene po celom korenu. Mađarska detelina (Trifolium pannonicum Jacq.) je najmlađi predstavnik roda Trifolium, sa potencijalom korišćenja u krmnoj proizvodnji usled niza pozitivnih osobina. Cilj ovog istraživanja bio je ispitivanje potencijala osam genotipova mađarske deteline (deo kolekcije semena Instituta) za simbiozu sa autohtonim sojevima rizobiuma na osnovu broja i morfoloških karakteristika nodula.
Primarily protein fractions by CNCPS (Cornell Net Carbohydrates and Protein System) of apple pomace silages
(Research Institute of Mountain Stockbreeding and Agriculture (RIMSA), 2022) Marković, Jordan; Lazarević, Đorđe; Racić, Nedeljko; Stepić, Marija; Bekčić, Filip; Kostić, I.; Vasić, Tanja
Apple pomace is a nutrient that can be included in a ruminant meal, as fresh, dried and ensiled. Drying the pomace would contribute to its inclusion in the concentrate mixture or as a single feed. The aim of this study was to quantify the primarily protein fractions by CNCPS (Cornell Net carbohydrate and Protein System) in apple pomace silages ensiled with dry beet pulp, sunflower meal and sugar beet pulp with inoculant andwithout inoculant. Apple pomace (AP) was obtained from the factory Vino Župa Aleksandrovac. Study treatments in the present research of the apple pomace sillage were: added feed (A) and applied inoculant (B): A1- ensiled apple pomace 100% participation (a1b1-apple pomace without inoculants; a1b2-apple pomace with inoculants; A2- apple pomace 85%+15% of dry beet pulp (a2b1-without inoculants; a2b2 with inoculants); A3-apple pomace 85%+15% sunflower meal (a3b1-without inoculants and a3b2 with inoculants); A4-apple pomace 84%+15% sugar beet pulp+Benural S 1% (a4b1-without inoculants and a4b2 with inoculants). Ensiled apple pomace with sunflower meal had the highest TP content, but the lowest NPN content of CP. Ensiled apple pomace with dry beet pulp had the highest IP, but the lowest SolP of CP. Treatments with inoculants had lower NDICP and ADICP than treatments without inoculants
Response of Different Perennial Ryegrass Varieties to Water Stress
(MDPI AG, 2025-04-22) Prijović, Mladen; Sokolović, Dejan; Maksimović, J. D.; Maksimović, V.; Milosavljević, D.; Babić, Snežana; Stepić, Marija; Sabovljević, A.
Perennial ryegrass represents the most important forage grass, yet its generally low drought tolerance leads to reduced yields under water scarcity. Nevertheless, large intra- and inter-population variability could be a pool for selecting new drought-tolerant varieties. In this study we evaluated three populations (K-11, Exp population and Shandon) under semi-controlled conditions across four watering levels (100%, 70%, 50% and 30% of field water capacity), focusing on yield and key morphological and biochemical traits. Dry matter yield and root dry mass decreased in all populations under limited watering conditions. The highest biomass production in such conditions was observed in the Exp population, likely due to better root performance in the deeper soil layer. On the other hand, oxidative stress markers (MDA and H2O2) and water-soluble sugars, which indicated the best physiological status in cultivar K-11 under severe drought, did not lead to the highest DMY. These results show the importance of including multiple physiological and biochemical traits in breeding processes, with the aim of developing perennial ryegrass cultivars capable of withstanding prolonged and intense summer drought as a consequence of climate change.
Seed size and protein content in dry grains of the faba bean (Vicia faba L.) lines originated from Serbian local populations
(LiveSeeding, the Polytechnic University of Coimbra, and supporting organisations and projects, 2025-05-26) Milenković, Jasmina; Petrović, Mirjana; Stepić, Marija; Bekčić, Filip; Andjelković, Snežana; Zornic, Vladimir; Perović, D.
In the past, the faba bean (Vicia faba L.) was an important legume in the Mediterranean diet for both humans and livestock. Today, however, it has largely been forgotten in Serbia. The Serbian Ministry of Agriculture currently recognizes only two commercial varieties of faba bean for animal feed (var. minor). Faba beans intended for human consumption are grown on very small plots of land, primarily for family use. The key factor for the survival of tiny local faba bean populations is the traditional preparation of faba bean tied to Lenten practices observed by Orthodox Christians. During this time, guests are offered a vegan, hummus-style dish made from faba beans. However, this dish is not prepared in all regions of Serbia, or every village. Seeds are infrequently transferred between farmers and are rarely found at the farmer’s markets, so it is feasible to believe that faba bean biodiversity in Serbia is high because maybe every farmer, which produces faba bean, has his own population. The Institute for Forage Crops Kruševac (IKBKS) has launched a research program to collect and evaluate native faba bean populations in Serbia. This collection now has approximately 30 original populations and about 60+ inbreed derived lines. According to preliminary assessments, the grain quality and grain bioactive material content are both a quite high (Milenković et al., 2024, Mitić et al., 2024). In this research, the lines were divided into two groups depending on seed size: major and equine-minor, to evaluate if grain size affects protein content. Because in Serbia the majority of local populations have a major grain size, the lines with equine and minor grain types are joined. The two-year experiment was conducted on the IKBKS plot in two repetitions using a randomized block system. The crude protein content was chemically analyzed using the Kjeldahl method. In this exploratory study, the crude protein content of the dry grain of faba bean lines was investigated in order to choose material for future work on the development of a new high-protein faba bean variety. According to the findings of this study, the protein content of the examined lines ranged between 26-31%, indicating that there is need for future research into the development of high-quality varieties for human or animal consumption. Low-input agriculture is consistent with sustainable farming goals in Serbia, and it can be especially beneficial for smallholder farmers trying to save costs while minimizing their environmental impact. Collecting and cultivating neglected and old species in conventional and organic agriculture can make a significant contribution to healthy food.
The response of different cultivars birdsfoot trefoil (Lotus corniculatus L.) on pre-sowing inoculation
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2022-10-06) Andjelković, Snežana; Babić, Snežana; Milenković, Jasmina; Stepić, Marija; Marković, Jordan; Bekčić, Filip; Mitra, D.
Birdsfoot trefoil (Lotus corniculatus L.) is a widespread plant species, which is important in providing sufficient quantities of quality fodder on soils of low production potential. Like many other forage legumes, this plant species has a well developed symbiosis with rhizobia. The aim of this study was to evaluate the effect of pre-sowing inoculation on height and green mass of birdsfoot trefoil. The experiment was a two-factorial design, where the growth of plants influenced by three variants of microbial inoculation (individual cultures of Rhizobium loti, azotobacter (Azotobacter chroococcum) and actinomycetes (Streptomyces spp.)) was compared with the growth of noninoculated controls for three cultivars (K-37, Rocco and Bokor). For this study, plants were sown on acid soil with pH 4.91. Measurements were performed three times during two years of research: one cut in the first year and two cuts in the second year. For tested parameters, interaction between cultivar and inoculation resulted in a different effect compared to the control. Highest plant height was achieved in cv. Rocco: in the first year using actinomycetes and in two cuts of the second year using azotobacter. In this study minimum height was recorded in cv. Bokor. By using actinomycetes, the highest value for green mass was achieved in cv. K-37 in the first year and in the first cut of the second year. In this cultivar, using azotobacter the highest green mass in the second cut of the second year was achieved.
Uticaj otkosa i faze razvića na razgradivost proteina lucerke i crvene deteline.
(Institut za krmno bilje Kruševac, Društvo za krmno bilje Republike Srbije, 2024-09-26) Marković, Jordan; Lazarević, Đorđe; Stepić, Marija; Racić, Nedeljko; Bekčić, Filip; Kistić, I.; Milosavljević, M.
Луцерка и црвена детелина представљају основни извор протеина, и један су од главних конституената оброка за преживаре. Ово место заузимају захваљујући способности биолошке фиксације азота и због њихове високе хранљиве вредности. Ове легуминозе су значајне јер битно смањују трошкове производње – мања примена азотних минералних ђубрива и мањи удео концентрованих хранива у оброцима за преживаре. Наведени бенефити и могућност да се исхрана животиња на фармама базира на кабастој храни привукли су пажњу бројних истраживача (Yu et al., 2003). Поред тога што се легуминозе одликују високом хранљивом вредношћу, њихови протеини подлежу брзој и интензивној деградацији у бурагу. Последично, стопа деградације протеина у бурагу директно утиче на ефикасност искоришћавања азота од стране животиња. Бројни подаци у литератури указују да и микроорганизми бурага и протеазе у биљнм материјалу узрокују неефикасно искоришћавање азота у организму животиња, што повећава ризик од губитка азота и његово излучивање у спољашњу средину (Kingston-Smith et al., 2003). Савремени системи исхране, у циљу минималних губитака азота подразумевају познавање количине и односа разградивих и неразградивих протеина у бурагу, као и неопходних количина азота за довољан развој микроорганизама. У току реализације постављених циљева извршена су истраживања на огледном пољу Института за крмно биље Крушевац, а испитиван је садржај разградивих и неразградивих протеина у сувој материји крушевачке сорте луцерке К-28 и крушевачке сорте црвене детелине К-39 у зависности од фазе развића и откоса.
Wilt of red clover caused by Fusarium oxysporum
(Palacký University Olomouc, 2023-09-10) Bekčić, Filip; Stepić, Marija; Andjelković, Snežana; Marković, Jordan; Vučković, N; Vico, I; Duduk, Natasa
Red clover (Trifolium pratense L.) is an important forage legume in Serbia, grown in many regions, especially due to its ability to grow on acid soils. Red clover plants with symptoms of stunting, slight chlorosis and necrosis of leaves, discoloration and partial root rot were sampled in the field where red clover is grown in monoculture in Ćuprija, Serbia, in July, 2021. Several isolates were obtained from the roots and one (F16A) was further identified and characterized based on morphological, molecular and pathogenic characteristics.
Багремац (Amorpha fruticosa L.) страна инвазивна врста на простору Специјалног резервата природе „Осредак”
(Српско биолошко друштво „Стеван Јаковљевић” Крагујевац, 2023, 2023-09-20) Petrović, Mirjana; Prijović, Mladen; Stepić, Marija; Andjelković, Snežana; Babić, Snežana; Zornic, Vladimir; Sokolović, Dejan
Специјални резерват природе „Осредак” је подручје од 250 ha површине које се простире на обе обале Западне Мораве, захватајући примарно атаре села Кукљин и Бела Вода тј. простор општина Крушевац и Трстеник. Овај простор је стављен под заштиту 2020. године Уредбом владе и управа је додељена ЈКП „Крушевац”. За потребе мониторинга биљних врста ангажовани су истраживачи Института за крмно биље Крушевац. Мониторинг се обавља од 2021. године у континуитету до сада и обухватао је сагледавање стања свих вегетацијских целина унутар резервата као и праћење заштићених и инвазиних врста.
Примена предсетвене инокулације луцерке на киселом зељишту
(Институт за крмно биље, Крушевац Друштво за крмно биље Републике Србије, Крушевац, 2024-09-26) Andjelković, Snežana; Babić, Snežana; Milenković, Jasmina; Bekčić, Filip; Stepić, Marija; Racić, Nedeljko; Radović, Jasmina
Захваљујући високом потенцијалу за принос и квалитет биомасе луцерка представља једну од најцењенијих крмних легуминоза. У симбиотској заједници ризобијум – луцерка фиксира се од 35 до 400 kg азота по хектару годишње. На теренима са киселим земљиштем услед слабо успостављене симбиотске асоцијације са квржичним бактеријама количина фиксираног азота и приноси луцерке су ниски, тако да се препоручује примена инокулације сојевима ризобиума толерантним на киселост. Циљ ових истраживања био је да се испита ефекат инокулације микроорганизмима који имaју улогу азотофиксатора и биостимулатора на параметре корена луцерке и то на: дужину (cm) и масу корена (g), број и масу нодула (g). Оглед је изведен у судовима у полуконтролисаним условима. Истраживања су вршена на сорти луцерке К-28 која је гајена на киселом земљишту (рНKCl 4,99). За инокулацију семена и земљишта употребљени су селекционисани сојеви ризобиума, азотобактера и актиномицета, при чему је ризобиум аплициран као моновалентни инокулум, али и као бивалентни инокулум у комбинацији са азотобактером, као и са актиномицетама. Такође, један третман је поливалентни инокулум који је садржао ризобиум, азотобактер и актиномицете. Контролна варијанта била је без инокулације. Анализе су вршене у фази почетка цветања луцерке, седам недеља након сетве.