Browsing by Author "Andjelković, Snežana"

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A rapid and cost-effective procedure for delineation and utilization of genomic microsatellites for paralleled genotyping in Vicia faba
(Czech Academy of Agricultural Sciences, 2015-03-31) Aleksic, Jelena M; Banović Đeri, Bojana; MILJUŠ-ĐUKIĆ Jovanka; JOVANOVIĆ Živko; Mikić, Aleksandar; Ćupina, Branko; ZLATKOVIĆ Bojan; Andjelković, Snežana; Spanu, Ilaria; Jelić, Mihailo; MAKSIMOVIĆ Vesna
Actinobacteria-enhanced plant growth, nutrient acquisition, and crop protection: Advances in soil, plant, and microbial multifactorial interactions
(Elsevier BV, 2022-02) MITRA; MONDAL; KHOSHRU; SENAPATI; RADHA; MAHAKUR; UNIYAL; MYO; BOUTAJ; SIERRA; PANNEERSELVAM; GANESHAMURTHY; Andjelković, Snežana; VASIĆ; RANI; DUTTA; MOHAPATRA
ANTIFUNGAL ACTIVITY OF RHIZOBIA AGAINST RED CLOVER PATHOGENS FROM GENUS FUSARIUM
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia University of Belgrade, Faculty of Agriculture, Serbia Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB) Italy, 2023-10-05) Bekčić, Filip; Duduk, Natasa; Lugić, Zoran; Radović, Jasmina; Babić, Snežana; Stepić, Marija; Andjelković, Snežana
Red clover (Trifolium pratense L.) is an important forage legume that can be found in nature or as cultivated crop in monoculture or in grass-legume mixtures. The great importance of red clover is the fact that it lives in symbiosis with Rhizobium leguminosarum bv. trifoli. In addition to promoting plant growth, productivity and biomass, the bacteria also have an important role in disease control. Apart from useful microorganisms, many soil pathogenic microorganisms inhabit red clover roots and under favorable conditions they cause infections and diseases of the cultivated plants. On the infected red clover plants Fusarium spp. can cause several symptoms: vascular wilt on older plants, dumping of seedlings, root rot, rot of root base and crown rot. In the present study, the antagonistic potential was evaluated for two bacteria isolates preliminarily determined as R. leguminosarum bv. trifoli (CD1 and CD6) against three isolates Fusarium spp. (G1, G2 and G3) who were obtained from the roots of symptomatic red clover plants. The research was performed in in vitro agar plate (PDA medium) by methods of dual culture (“bacterial ring”). The comparison of growth assessment of fungi after they were grown together with rhizobia in a single plate and growth of fungi in individual plates was performed. Both rhizobia isolates showed significant antifungal activity against all the tested phytopathogenic fungi. Growth inhibition of G1, G2 and G3 was evaluated (%) using both rhizobia isolates, CD1 varied from 30 to 43 % and CD6 from 14 to 32 %.
Antioxidant Activities, Total Phenols, and Proanthocyanidin Changes during Storage of Fourteen Faba Bean (Vicia faba L.) Populations from Serbia – A Chemometric Approach
(Informa UK Limited, 2023-06) Mitic, Violeta; Nikolić, Jelena; Andjelković, Snežana; Petrović, Mirjana; Stankov Jovanovic, Vesna; Milenković, Jasmina
Considering the interest in the consumption of faba bean and study of antioxidants in natural food resources, methanol extracts of fourteen Vicia faba cultivars harvested from a two-year field experiment were studied for their phenolic compounds, proanthocyanidines, and antioxidant activities by the DPPH free radical scavenging assay, ABTS cation radical decolorization assay, ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), and total reducing power (TRP). The results indicate significant differences among the populations in their content of polyphenols and proanthocyanidine, resulting in variations in antioxidant activity of seed extracts. The storage of V. faba mature seeds under uncontrolled, but identical conditions decreased the total phenol content and antioxidant properties. The total phenols were from 10.12 ± 0.19 to 18.53 ± 0.14 mg GAE g−1 DE (2021 year) and 19.52 ± 0.16 to 26.94 ± 0.34 mg GAE g−1 DE (2022 year). In the 2022 growing season, the DPPH free radical scavenging activity had higher values for all populations from 4.83 ± 0.002 to 6.17 ± 0.003 g mg TE g−1 DE. Hierarchical cluster analysis and principal component analysis obtained from antioxidative profiles grouped the samples into two major clusters. The first cluster consists of extracts of V. faba cultivars from the 2021 growing season, while the V. faba cultivars from the 2022 growing season are in second cluster. Tested cultivars can be used as a readily available source of natural antioxidants.
Autochthonous plant growth-promoting rhizobacteria enhance Thymus vulgaris growth in well-watered and drought-stressed conditions
(Lithuanian Research Centre for Agriculture and Forestry, 2021-12-06) Stamenov, Dragana; Djuric, Simonida; Hajnal Jafari, Timea; Andjelković, Snežana
Cell Wall Components of Italian Ryegrass Depending on the Harvest and Application of Nitrogen Fertilizer
(Research Institute of Mountain Stockbreeding and Agriculture, Troyan, 2023-10-01) Marković, Jordan; Petrović, Mirjana; Lazarević, Đorđe; Andjelković, Snežana; Zornic, Vladimir; Vasić, Tanja; Štrbanović, Ratibor
The importance of perennial grasses is multiple. They represent the basis of sustainable animal husbandry and the basis of the animal feed industry, forming the staple food of ruminants in areas with a moderate climate as components of natural and sown grasslands, natural and sown meadows that can be used through mowing, haylage and silage. Adequate and advanced management of perennial grasses results in low prices offorages, significantly lower than concentrated feed. Italian ryegrass is characterized by rapid development and production of a large amount of quality forage crops.
EFFECT OF AZOTOBACTER SPP. ON INITIAL GROWTH OF GRASSES
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia University of Belgrade, Faculty of Agriculture, Serbia Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB) Italy, 2023-10-05) Andjelković, Snežana; Sokolović, Dejan; Lugić, Zoran; Jevtić, Goran; Radović, Jasmina; Milenković, Jasmina; Babić, Snežana
The composition of the microbial community in rhizosphere can affect plant growth, nutrients uptake, and stress tolerance. In rhizosphere bacteria belonging to the genus Azotobacter there are free nitrogen-fixers promoting growth and health of plants. The aim of this research was to investigate the effect of different native isolates (SA74, SB94, MA7 and their combination) Azotobacter spp. on initial growth of plants tall fescue (Festuca arundinacea Scherb) and meadow fescue (Festuca pratensis Huds). The experiment was carried out in semi-controlled conditions. For this study, seeds of tall fescue cultivar Kruševački 20 (K-20) and meadow fescue cultivar Kruševački 21 (K-21) were planted in pots filled with alluvial soil with slightly acidic reaction. The height, green mass per plant, root length and root weight per plant were determined. Four variants of microbial inoculation were compared with the growth of non-inoculated control. The significance of difference between examined treatments was determined by Fisher’s LSD test (p<0.05). At tall fescue, applied isolate SB94 in all investigated parameters had a negative effect. The two other isolates as well as combination of three isolates had a positive effect on initial growth of plants. Only in plant height, the application of a combination of isolates did not have positive result compared to the control. At meadow fescue, inoculation with combined isolates positively influenced plant height andgreen mass per plant. Root length was significantly increased only in treatment with isolate MA7. The influence of inoculation on root weight per plant was not noted.
Effect of Nitrogen Fertiliser and Lime on the Floristic Composition, Soil Microbes and Dry Matter Yield of Danthonietum calycinae Grassland
(University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 2019-11-08) Zornic, Vladimir; Stevović, Vladeta; Lugić, Zoran; Andjelković, Snežana; Jevtić, Goran; Radović, Jasmina; Petrović, Mirjana
Natural grasslands are significant resources for forage production, which is not exploited enough, mostly because of low production, as a result of bad grasslands management. The research has been carried out on the association Danthonietum calycinae to evaluate the effects of different nitrogen application rates (N20, N80 and N140) and lime (1 tha-1) on the floristic composition, soil microbes and dry matter yield. Botanic composition was strongly influenced by the treatments, especially by N applications. Nitrogen fertilisation led to significant increase of grass species in the association (from 57% to 82%), while higher rate of nitrogen caused a significant decrease of plants from other families (especially legumes). Application of low and medium rate of nitrogen positively effect on microbial abundant in soil, but the highest dose (140 kg N) reduced all microbial count, except fungi. Mineral nitrogen fertilisation had a favourable effect on dry matter yield in all treatments. The highest increase in dry matter yield relative to the control was in N140 treatments (6.66 t ha-1). Results indicated that optimal applications of nutrients for this association is application of PK and lime and 80 kg of nitrogen which enable high yield (6.38 t ha-1) in comparison with control (3.16 t ha-1) and preserving soil fertility and the environment at the same time.
ENVIRONMENTAL ASSESSMENT OF SOME HEAVY METALS IN SELECTED MEDICINAL PLANT SPECIES ALONG A BUSY ROAD IN VRNJAČKA BANJA, SERBIA
(Asociatia Carpatica de Mediu si Stiintele Pamantului, 2022-08-31) BABIĆ; MITRA; DAS MOHAPATRA; PAPOVIĆ; Milenković, Jasmina; Jevtić, Goran; Babić, Snežana; Andjelković, Snežana
This study reported the concentrations of Pb, Cd, Cr, Mn in parts of four medicinal plant species (Cichorium intybus L., Mentha×piperita L., Plantago lanceolata L., Verbascum densiflorum Bertol.) collected from two sites, one few meters away and the other 500 m away from a busy road in Vrnjačka Banja, Serbia. Heavy metals were determined by the atomic absorption method using atomic absorption spectrophotometer. Similar content of tested metals in the soil of the tested locations was recorded, only there was more manganese in the soil closer to the road. The highest concentrations of heavy metals were found in the roots of plants closer to the road. The leaves and stem contain smaller quantities of these metals than root, but there are differences between the studied plant species. In conclusion, all examined metals were present in higher concentrations in plants at the location closest to the road than at the location further away from the road.
Forage Cultivation Under Challenging Environment
(Springer Nature Singapore, 2023) Milenković, Jasmina; Petrović, Mirjana; Andjelković, Snežana; Mitra, Debasis
We already experience the effects of climate change, and future agricultural output will face a number of challenges. High temperatures, scarcity of water, rising pollution, an increase in weeds and pests are just a few of the negative effects it may have. The area and quality of arable land are both decreasing as a result of climate change. It will not be able to generate adequate feed for animals since the negative effects of climate change will reduce the output of fodder plants. Reduced human food production is the eventual result. Every stage of plant growth is impacted by environmental factors (planting, seed germination, early vegetative growth, flowering, maturing and production of seeds, wintering, seed storage, etc.). Some forage crops could be grown and cultivated on less productive soils and extreme environmental conditions, and this could be their advantage. In order to produce and use forage crops more effectively, new species that are more tolerant to stressful situations must be introduced, as well as wild relatives of conventional species and microbiological fertilizers containing rhizobia and other microorganisms. Plant-based proteins have recently gained popularity as a source of protein, and as many forage plants have a high protein content, they can be used in this quickly expanding industry.
Forage quality of different Festulolium cultivars
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2022-10-06) Babić, Snežana; Sokolović, Dejan; Andjelković, Snežana; Petrović, Mirjana; Jevtić, Goran; Prijović, Mladen; Racić, Nedeljko
Hybrids created by crossing between genera Festuca and Lolium species are Festulolium.Crossing species of these genera aim to combine their positive characteristics, such as Festuca tolerance to abiotic stresses and edaphic and climatic conditions with the high quality and digestibility of Lolium perenne and Lolium multiflorum species. Forage quality of 15 different Lolioid Festulolium cultivars and six cultivars of Festuca and Lolium pure species were investigated in a moderate continental climate in Kruševac, Serbia. Plots in the trial (7,5 m2 ) were completely randomized, each in three replications. In the year of establishment, the plots were cut without weighing and taking samples. In the next two years, two cuts were taken. The first cut was done at the beginning of the heading (the first half of May) and the second in the first half of July. On dry samples, from two cuts, the content of crude protein, crude cellulose, crude fat, ash, ADF, NDF and ADL was determined by standard laboratory methods. Results were presented as two-year average values. The best dry matter quality of Festulolium cultivars was determined for cultivars AberNiche and Felopa. The highest crude protein content in the first cut was determined for AberNiche (187.1 gkg-1 ) and Felopa (169.6 gkg-1). Also, the same cultivars noted the lowest values for ADF (296.7 and 303.5 gkg-1 ) and for AberNiche was determined the lowest ADL contet (35.4 gkg-1 ) in the first cut. These cultivars are in the group of cultivars with the lowest NDF value. Based on the obtained results, it can be concluded that the analyzed Festulolium cultivars have a higher crude protein content compared to the cultivars of Festuca pure species, but lower than Lolium species.
Heritability and variability of the most important traits of meadow fescue synthetic cultivars
(Agricultural Research Ltd. Troubsko, Czech Republic, 2023-09-10) Babić, Snežana; Radović, Jasmina; Andjelković, Snežana; Petrović, Mirjana; Jevtić, Goran; Prijović, Mladen; Sokolović, Dejan
Meadow fescue (Festuca pratensis Huds.) is one of the most important perennial grasses for animal feed production on grasslands. It is characterized by high and stable yield potential and good biomass quality. The aim of this study was to determine the broad and narrow sense heritability and genetic (CVG%) and phenotypic (CVF%) coefficient of variation of the most important traits of two synthetic cultivars (Kruševački 21 and Pradel).
Influence of stimulative feeding measures on spring development of bee societies in DB and LR honeycombs
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2022-10-06) Jevtić, Goran; Babić, Snežana; Andjelković, Snežana; Zornic, Vladimir; Lazarević, Đorđe; Matovic, Kazimir; Nedić, N.
In order for the bee colonies to make the most of the main pasture, and primarily black locust, it is very important that they are in good condition. It is necessary for the societies to reach their maximum strength at the beginning of the main pasture, and not for it to serve them for further development. The beekeeper himself plays a crucial role in the accelerated spring development of bee colonies. The aim of this study was to determine the effect of different ways of spring feeding on the strength of bee colonies and the food supply in them. The experiment was performed with companies that were in Db (Dadant-Blatt) and Lr (Langstroth-Ruth) hives. Four groups of companies were tested, and there were five companies in each group. The first group was supplemented with sugar syrup, enriched with vitamin-mineral complex - Foprsapine (10 drops per liter of syrup). The second group of societies was fed with honey dissolved in water (ratio of honey and water 1:1). The third group of the companies was fed with sugar dough (energy cake without additives). The fourth group of companies was supplemented with sugar syrup (the ratio of water and sugar was 1:1). At the beginning of the experiment, the societies in Lr hives had an average of 4.5 frames with bees, and 2.5 frames with a brood. In the Db hives, the societies occupied five frames with bees, and had 2.7 frames with a brood. At the end of the experiment Lr societies had 8.7 frames with bees, and 4.5 frames with brood, while Db societies had nine frames with bees, and 4.7 frames with brood. The experiment was performed at the apiary of the Institute for Forage Crops in Kruševac.
INSTITUT ZA KRMNO BILJE, KRUŠEVAC, 140 GODINA U SLUŽBI RAZVOJA POLJOPRIVREDE
(Centar za strna žita i razvoj sela Kragujevac, 2023-06-22) Lugić, Zoran; Sokolović, Dejan; Radović, Jasmina; Babić, Snežana; Milenković, Jasmina; Andjelković, Snežana
Institut za krmno bilje u Kruševcu je akreditovani istraživačko razvojni institut. Formiran je 1884. godine, kao Ugledno dobro i vrt, koje je imalo za cilj unapređenje ukupne poljoprivredne proizvodnje. Od 1959. godine, bavi se isključivo istraživanjem krmnog bilja. Glavna istraživanja u Institutu su proučavanje i očuvanje postojećih genetičkih resursa, unapređenje genetičkog potencijala krmnih biljaka, tehnologija proizvodnje i konzervisanja krmnog bilja na oranicama i travnjacima, a sve u cilju unapređenja stočarske proivodnje. Do sada je u Institutu stvoreno preko 40 novih sorti krmnih vrsta. U poslednjih deset godina odbranjeno je 8 doktorskih disertacija, objavljeno preko 250 naučnih radova i saopštenja, a istraživači Instituta su učestvovali, pored nacionalnih i u međunarodnim projektima (EUREKA, TEMPUS, HORISON), što ukazuje da institut ima značajno mesto u domaćoj i međunarodnoj naučnoj zajednici. U saradnji sa Ministarstvom poljoprivrede i lokalnim samoupravama, realizovani su projekti navelikom broju gazdinstava, čiji je cilj primena dobijenih rezultata istraživanja u praksu. Dugogodišnja tradicija, iskustvo i dosadašnji rezultati čine da je Institut ustanova koja je i dalje u službi razvoja poljoprivrede, kojoj poljoprivredni prozvođači veruju.
Morphological and Chemical Characterization of a Collection of Mountain Clover Natural Populations
(Institutul Național de Cercetare Dezvoltare Agricolă Fundulea, 2022) Petrović, Mirjana; Lugić, Zoran; Zornic, Vladimir; Andjelković, Snežana; Tomić, Dalibor; Milenković, Jasmina; Jevtić, Goran
With climate change evident, the possibility opens up of introducing into production a species that, although not characterized by high yield, nevertheless offers some other benefits for both the environment and man. One of these species is mountain clover (Trifolium montanum L.), a species widespread around European in the past, but due to agricultural activities its habitat has become fragmented and areas of mountain clover impaired. In the present study, the collection of nine natural populations of mountain clover originating from different parts of the hilly-mountainous areas of Serbia was tested in field conditions. We analysed different morphological traits (green plant biomass, stem length, internode number, number of lateral branches, leaf length and leaf width), dry matter quality traits (content of crude proteins, crude fibre and crude fat) as well as secondary metabolites (total phenolic content, flavonoid contents and antioxidative activity). We collected morphological data and plant samples during 2011 and 2012. We performed descriptive statistics to provide basic information about variables in the dataset, then calculated Shannon-Weaver diversity index (H’) and performed two-way ANOVA and principal component analyses (PCA). Analysing the broad range of data collected during two years, we found considerable morphological and chemical diversity amongst the collection of mountain clovers from central Serbia. Mean coefficient of variation (CV) in the morphological dataset ranged from 18% (stem length) to 57.6% (plant biomass) in 2011 and from 16.5% (leaf length) to 70.6% (stem number) in 2012. Dry matter (DM) parameters displayed the lowest CV, ranging from 6.1% (crude proteins) to 14.8% (crude fat), indicating that these parameters were less discriminative within the study collection. Over all populations, average crude protein content was 19.5%, and average crude fibre content was 27.3%. Total phenolic contents (expressed as gallic acid equivalent, GAE) ranged from 49.8 to 89.7 mg GAE g-1 DM, and flavonoid contents (expressed as rutin, Ru, equivalent) ranged from 66.8 to 142 mg Ru g-1 DM. Average antioxidative activity expressed in terms of IC50 values ranged from 177 to 426 mg ml-1 of methanol extract.
OPLEMENJIVANJE VIŠEGODIŠNJIH TRAVA U SRBIJI
(Centar za strna žita i razvoj sela Kragujevac, 2023-06-22) Babić, Snežana; Sokolović, Dejan; Radović, Jasmina; Andjelković, Snežana; Petrović, Mirjana; Jevtić, Goran; Prijović, Mladen
Višegodišnje trave kao baza održivog stočarstva i industrije stočne hrane, predstavljaju osnovnu hranu preživara u oblastima umerenog klimata. Kao komponente prirodnih i sejanih travnjaka koriste se putem ispaše, kosidbe, senaže i silaže. Najvažnije vrste za stočnu hranu su: ježevica, engleski ljulj, livadski vijuk, visoki vijuk, mačji rep, italijanski ljulj, francuski ljulj, crveni vijuk i bezosni vlasen. Genotip, odnosno sorta u prinosu višegodišnjih trava učestvuje i više od 50%. Najbitniji preduslov uspešnog oplemenjivanja je visok stepen genetičke varijabilnosti u početnom oplemenjivačkom materijalu. Osnovne osobine na koje se vrši selekcija su prinos i kvalitet suve materije, vreme stasavanja, perzistentnost i tolerancija na sušu i prinos semena. Najčešće korišćena metoda oplemenjivanja je fenotipska rekurentna selekcija, modifikovana u cilju skraćenja ciklusa selekcije i redukcije nepoželjnih ukrštanja, praćena polikros metodom ukrštanja i formiranjem sintetičke sorte. Dugogodišnjim oplemenjivačkim radom u Institutu za krmno bilje Kruševac je stvorena 21 krmna i 1 parkovska sorta višegodišnjih trava.
PREDSELEKCIONO ISPITIVANJE GENOTIPOVA MAĐARSKE DETELINE
(Društvo genetičara Srbije, Beograd, 2023-10-16) Petrović, Mirjana; Zornic, Vladimir; Milenković, Jasmina; Stepić, Marija; Radović, Jasmina; Tomić, Dalibor; Andjelković, Snežana
Vrste roda Trifolium ostvaruju simbiotsku vezu sa zemljišnim bakterijama, pripadnicima vrste Rhizobium leguminosarum bv. trifoli koja se ogleda u formiranju sitnih kvržica koje su raspoređene po celom korenu. Mađarska detelina (Trifolium pannonicum Jacq.) je najmlađi predstavnik roda Trifolium, sa potencijalom korišćenja u krmnoj proizvodnji usled niza pozitivnih osobina. Cilj ovog istraživanja bio je ispitivanje potencijala osam genotipova mađarske deteline (deo kolekcije semena Instituta) za simbiozu sa autohtonim sojevima rizobiuma na osnovu broja i morfoloških karakteristika nodula.
PRISUSTVO AKTIVNIH MATERIJA U GRAHORICI
(Društvo genetičara Srbije, Beograd, 2023-10-16) Milenković, Jasmina; Andjelković, Snežana; Petrović, Mirjana; Zornic, Vladimir; Radović, Jasmina
Grahorica (Vicia sativa spp.) se može koristiti u ljudskoj ishrani jer, pored visokog sadržaja proteina i drugih hranljivih materija, može imati i zdravstvene koristi. Međutim, u grahorici se nalaze jedinjenja poznata kao antinutritivni faktori (NNF – non-nutrient factors) koji mogu smanjiti iskoristljivost ostalih hranljivih materija i negativno uticati na zdravlje. Sa druge strane, NNF, u određenoj koncentraciji, mogu imati određene zdravstvene prednosti jer omogućavaju bioraspoloživost esencijalnih minerala i pozitivno utiču na biohemijske procese u organizmu. Novija istraživanja su fokusirana na ispitivanje koncentracije ovih materija koje bi omogućile ravnotežu između korisnih i štetnih efekata, da bi se dobila poželjna interakcija sa drugim komponentama ishrane.
Rock Phosphate Solubilizing Potential of Soil Microorganisms: Advances in Sustainable Crop Production
(MDPI AG, 2023-05-10) Trifunović, B; Nosratabad, A.F.; Mitra, D.; Chaithra, M.; Danesh Y.R.; Boyno, G.; Chattaraj, S.; Priyadarshini, A.; Andjelković, Snežana; Pellegrini, M.; Guerra-Sierra, B.E.; Sinha, S.
Phosphorus (P) is one of the most important elements required for crop production. The ideal soil pH for its absorption by plants is about 6.5, but in alkaline and acidic soils, most of the consumed P forms an insoluble complex with calcium, iron, and aluminum elements and its availability for absorption by the plant decreases. The supply of P needed by plants is mainly achieved through chemical fertilizers; however, in addition to the high price of these fertilizers, in the long run, their destructive effects will affect the soil and the environment. The use of cheap and abundant resources such as rock phosphate (RP) can be an alternative strategy for P chemical fertilizers, but the solubilization of P of this source has been a challenge for agricultural researchers. For this, physical and chemical treatments have been used, but the solution that has recently attracted the attention of the researchers is to use the potential of rhizobacteria to solubilize RP and supply P to plants by this method. These microorganisms, via. mechanisms such as proton secretion, organic and mineral acid production, siderophore production, etc., lead to the solubilization of RP, and by releasing its P, they improve the quantitative and qualitative performance of agricultural products. In this review, addressing the potential of rhizosphere microbes (with a focus on rhizobacteria) as an eco-friendly strategy for RP solubilization, along with physical and chemical solutions, has been attempted.
Short communication: Morphological description and molecular detection of Pestalotiopsis sp. on hazelnut in Serbia
(Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA), 2017-07-10) Vasic, Tanja; Jevremović, Darko; Krnjaja; Leposavić, Aleksandar; Andjelković, Snežana; Živković, Sanja; Paunović, Svetlana
In autumn 2015, hazelnut plants with leaf blight symptoms were noticed in a commercial plantation in the Province of Vojvodina, Serbia. Symptomatic samples were collected and submitted to laboratory analysis. Based on morphological characterization, the fungus isolated from the material was initially identified as Pestalotiopsis sp. Pathogenicity tests showed that two selected isolates infected hazelnut leaves and fruits that developed symptoms after artificial inoculation. The pathogen was re-isolated from diseased leaves and fruits, confirming Koch’s postulates. Molecular identification was performed with sequence and phylogenetic analysis of ITS, EF1-α, and TUB genomic regions. Phylogenetic analysis confirmed the results of the morphological identification. The detection of Pestalotiopsis sp., a causal agent of leaf blight on hazelnut in Serbia, is one of a few reports of these pathogenic fungi on hazelnut.