Bošković-Rakočević, LjiljanaPavlović, NenadMladenovic, JelenaMarjanović, MilošZdravković, JasminaTošić2024-07-172024-07-172023-06-012079-05382079-052X10.9755/ejfa.2023.3128https://rifoc.ikbks.com/handle/123456789/80The aim of this research was to determine the effect of the application of different types of nitrogen fertilizer in five different varieties of lettuce on yield. The experiment was performed in the open field, in the spring cycle of growing during 2018 and 2019. The size of the base plot was 3 m2, and the trial was set up in a random block system. The trial covered the application of various forms of nitrogen fertilizers: ammonium sulphate, ammonium nitrate and urea. The applied nitrogen dose is 100 kg/ha (May 10th 2018 and May 8th 2019). Samples for the analysis of vitamin C and nitrate content, as well as yield determination, were carried out at the stage of the technological maturity of lettuce (60-62 days after seedling). The average weight of lettuce, depending on the variety, varied in the range of 211.3 g to 258.7 g. The use of ammonium nitrate resulted in the highest average weight of lettuce head (246.7 g), followed by the use of urea (238.9 g) and the lowest by the use of ammonium sulphate (221.0 g). The nutritional value of lettuce (vitamin C content) shows that depending on the applied form of nitrogen in the fertilizer, the highest content of vitamin C was determined by urea (10.86 mg/100 g), then ammonium sulphate (9.61 mg/100 g), and the lowest by ammonium nitrate (7.38mg/100g). The lower content of vitamin C was found in varieties of lettuce with red leaves compared to varieties with green leaves. The accumulation of nitrate in lettuce leaves directly depends on the variety and the applied fertilizer. The highest nitrate content was with ammonium nitrate (2355.6 mg/kg). The accumulation of nitrate in lettuce varieties with red leaves was higher compared to varieties with green leaves. Keywords: Lettuce, Nitrate, Vitamin C, Yield, Open fieldarticle