Duboja Krstić, A.Novaković, M.Pešić, S.Stojanović, D.Stepić, MarijaJoković, N.Vitorović, J.2025-12-092025-12-092025-11-13978-99976-86-29-9https://rifoc.ikbks.com/handle/123456789/251Potentilla argentea L., is traditionally used in Kruševac, Serbia, as an herbal decoction for the treatment of gastrointestinal disorders. This study evaluated and compared the antimicrobial activity of both the plant root and aerial parts (stems, leaves and flowers). P. argentea samples were collected during two different periods: March and May. Samples were dried, ground, and subjected to ultrasonic extraction using methanol (ME) and water (AE). The aqueous extract of the root was prepared following the traditional method of tea preparation, with modifications involving 24-hour steeping in water prior to filtration, rather than immediate use as in typical preparation. Antimicrobial activity was assessed by determining Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) against seven American Type Culture Collection (ATCC) bacterial strains: Staphylococcus aureus ATCC 6538, Bacillus cereus ATCC 11778, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Salmonella enteritidis ATCC 13076, Enterobacter aerogenes ATCC 13048 and Pseudomonas aeruginosa ATCC 9027. None of the extracts inhibited E.coli, while the strongest antimicrobial activity was observed againts S. enteritidis (0,0098/0,0098 mg/ml). ME from aerial parts exhibited stronger antimicrobial activity compared to root extracts. However, root ME exhibited greater antimicrobial activity against B. cereus compared to aerial part ME, while it showed no antimicrobial activity against E. aerogenes and P. aerogenes. No significant difference was observed between root and aerial parts ME against S. aureus and E. faecalis, though samples harvested in May exhibited greater antimicrobial acitivity in both cases. AE from roots collected in March showed no antimicrobial activity.enCC0 1.0 Universalhttp://creativecommons.org/publicdomain/zero/1.0/silver cinquefoilMICMBCherbal decoctionultrasonic extractionconferenceObject