Vasić, TanjaŽivković, SanjaMarković, JordanFilipović, S.Debasis, M.2025-09-162025-09-162024-10-10978-99976-816-8-3https://rifoc.ikbks.com/handle/123456789/202Саопштење са међународног скупа штампано у целини (M 33)Alfalfa (Medicago sativa L.) occurs in nature as a cultivated and wild species. As one of the oldest and most important perennial forage crops, alfalfa has the potential to produce large amounts of high-quality forage. This paper presents the preliminary results of the population study of phytopathogenic fungi on alfalfa. A total of 625 samples of alfalfa plants were collected between 2002 and 2019 in Serbia from 17 locations. Standard phytopathological isolation was performed from the transition of healthy to diseased tissue on potato dextrose agar (PDA) with streptomycin. After incubation at 22°C and a light regime of 12 hours day/12 hours night, the obtained cultures were examined microscopically and the morphological identification of fungi to the genus level was carried out using standard keys. Checking the pathogenicity of the obtained isolates was done through artificial inoculation with damage to plant parts. Inoculation was performed with 7-day-old fungi isolates, and the inoculated plants were stored at a temperature of 20 to 25°C. Based on the morphological and pathogenic characteristics of the obtained isolates, it was determined that alfalfa pathogens were dominant in Serbia Colletotrichum, Fusarium, Verticillium, Rhizoctonia, Sclerotinia and Phoma. As the causative agents of alfalfa diseases are not sufficiently studied in Serbia, the conducted research represents a significant contribution to defining the role of phytopathogenic fungi in the symptoms they cause on alfalfa, all with the aim of contributing to finding adequate measures for their successful suppression.enCC0 1.0 Universalhttp://creativecommons.org/publicdomain/zero/1.0/alfalfaphytopathogenic fungi.conferenceObject