Zornic, VladimirLugić, ZoranRacić, NedeljkoAndjelković, SnežanaTomić, DaliborMarković, JordanLazarević, ĐorđePetrović, Mirjana2025-12-152025-12-1520251311-0489(Print)2367-8364(Online)https://rifoc.ikbks.com/handle/123456789/260The use of mineral fertilizers changes the floristic composition of grassland communities and increases productivity. However, fewer studies have been conducted on the medium-term (5--10 years) effects of mineral fertilizers on yield and floristic composition, particularly on changes in soil microbiom. After eight years (2012-2020), the effect of fertilizers (control -- no fertilizers, PK-60 kg ha-1 P2O5, 60 kg ha-1 K2O, N20PK-20 kg ha-1 N, 60 kg ha-1 P2O5, 60 kg ha-1 K20, N80PK-80 kg ha-1 N, 60 kg ha-1 P2O5, 60 kg ha-1 K20, and N140PK-140 kg ha-1 N, 60 kg ha-1 P2O5, 60 kg ha-1 K20) and liming (no lime and lime with 1 t/ha) on the grassland community of Danthonia alpina Vest (syn Danthonia calycina) were observed. We researched the effects treatments on productivity and share of grasses (Fam Poaceae), legumes (fam Leguminosae) and forbs (other species). Soil microbiome have been identified by analyzing the number of: total microbial count, fungi, free living nitrogen fixers and actinomycetes. PK treatments stimulated legumes, while grass cover decreased. Also increasing nitrogen added, grass cover abundance has risen. Liming significantly increased the total number of microorganisms and actinomycetes, while the number of fungi was significantly reduced. Treatment N80PK involved the greatest amount of microbes and fungus, whereas treatment PK contained the highest number of actinomycetes and free nitrogen fixers. According to the results, fertilizer treatments showed a significant increase yield and it gradually rose as applied nitrogen increased. The highest yield compared to the control (1,8 t ha-1) was achieved in N140PK (5,68 t ha-1), however, it was not significantly different to the N80PK (5,27 t ha-1). The yield was not significantly increased by raising the nitrogen content from 80 t ha-1to 140 t ha-1, but abundance of the most of examined groups of microorganisms significantly decreased, so the dose N140PK is not completely appropriate.enCC0 1.0 Universalhttp://creativecommons.org/publicdomain/zero/1.0/NPK fertilizersgrasseslegumesmicrobial countarticle