RIFoC
Digitalna archive of the Instiitute for Forage Crops
Primary research results available.
- Institutional digital repository of the Institute for Forage Crops established in December 2023.
- Since April 2024. portal eNauka regularily harvests meta-data from RIFoC repository.
Contents of RIFoC
- Ustanova vodi poreklo od Uglednog dobra i vrta formiranog 2. februara 1884. godine odlukom Podružine kruševačke Srpskog poljoprivrednog društva na Drugom glavnom zboru a “na osnovu tvrdog uverenja da se neposrednim primernim radovima na oglednim dobrima, najjače i najbrže može dejstvovati na unapredjenje poljoprivrede narodne”. U toku svoje istorije, menjani su nazivi i delimično nadležnosti, a od 21. oktobra 1932. godine postaje naučna ustanova spajanjem Agrobotaničkog odseka Poljoprivredne ogledne i kontrolne stanice u Topčideru i Poljoprivredne stanice u Kruševcu
Recent Submissions
Antioxidant potential of the methanol and water extract of Potentilla argentea L. aerial parts and roots
(Prirodno-matematički fakultet, Univerzitet u Banjoj Luci, 2025-11-13) Duboja Krstić, A.; Novaković, M.; Pešić, S.; Stojanović, D.; Stepić, Marija; Joković, N.; Vitorović, J.
Potentilla argentea L. is traditionally used in the municipality of Kruševac, Serbia, as tea to treat stomach ailments. In this study, aerial parts (stems, leaves and flowers) and roots of P. argentea were collected in two different growth stages near the village of Ribare, Serbia. The first period of collection was during vegetative growth at the end of March and the second during the flowering phase at the end of May. Dried aerial parts and root samples were subjected to ultrasonic extraction using methanol and water as solvents. Total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were determined, along with their antioxidative potential using DPPH, Ferric Reducing Antioxidant Power (FRAP) and Total Antioxidant Capacity (TAC) assays. The TPC and TFC of aerial parts and root samples were not significantly different when comparing the two growth stages. Root methanol extracts (RME) exhibited the highest TPC values followed by methanol extracts of aerial parts (MEAP) and root water extract (RWE). However, TFC was higher in MEAP compared to RME and RWE. Samples collected during flowering showed better antioxidative potential in DPPH, FRAP and TAC assays to those collected during vegetative growth. RME showed the strongest antiradical activity in the DPPH assay and also exhibited higher FRAP and TAC values than MEAP. Overall, the RME obtained from flowering P. argentea displayed the most pronounced antioxidant potential. Strong antioxidant potential demonstrated by the plant through multiple assays provides scientific support for its traditional use in the treatment of gastrointestinal disorders.
Expected genetic gain of the most important traits in the breeding process of the meadow fescue.
(2025-09-01) Babić, Snežana; Sokolović, Dejan; Prijović, Mladen; Andjelković, Snežana; Lazarević, Đorđe; Zornic, Vladimir; Radović, Jasmina
Meadow fescue (Festuca pratensis Huds.) is an important perennial forage grass for animal feed production on grasslands and pastures, with high yield potential and good-quality biomass. During the breeding process, it is often necessary to predict, based on the results of the initial selection cycles, how much a certain trait can be improved. To that end, it is necessary to determine the expected genetic gain (ΔGe), a parameter that tells us how much a trait can be improved by breeding it alone in the following period. Individual plants for hybridization, to obtain progeny by the method of Comstock and Robinson, were selected completely randomly. To examine the obtained progeny, the study was performed in an experimental field of the Institute for Forage Crops, in two replications. The experiment was set up according to the Nested Design I, with sets within the replications. In cv. Kruševački 21 (K-21), 60 full-sib progeny were studied in two sets, while in the cv. Pradel a total of 39 full-sib progeny were studied. Within each full-sib progeny, 60 plants were analyzed. In both cultivars, the highest expected genetic gain was determined for the number of generative and vegetative tillers per plant and dry matter yield per plant. The least success can be achieved by improving the heading date and crude protein content in the 1st cut in cv. K-21, and in cv. Pradel for traits leaf length and crude protein content in the 1st cut. By breeding individual traits, based on the obtained results, a large genetic gain is expected in many studied traits. Considering the existence of correlations between the most important agronomic traits, it is observed that often, by breeding one trait, a negative response is encountered in another trait. For this reason, the expected indirect genetic gain from selection was calculated, i.e., the effect of breeding one trait on other ordered traits. In the cv. K-21, achieving the expected genetic gain in plant height, would lead to a decrease in dry matter yield and leaf length. By creating later cultivars, the result would be a cultivar with a smaller number of generative tillers and a lower protein content in the first cut. Successful breeding for increased crude protein content in the first cut of 9.9 gkg-1 would result in a significant reduction in dry matter yield, followed by a reduction in the number of vegetative and generative tillers, but also earlier maturity. In cv. Pradel, a positive effect of the selection of individual traits on other studied traits was recorded for most of the traits. The only realization of the expected genetic gain in the increase in leaf length would result in a decrease in the number of vegetative tillers per plant.
Binucleate Rhizoctonia AG-A pathogen of red clover in Serbia
(2025-09-01) Bekčić, Filip; Milenković, Jasmina; Babić, Snežana; Stepić, Marija; Andjelković, Snežana; Duduk, N.; Vico, I.
Red clover (Trifolium pratense L.) in Serbia has a long history of cultivating due to its favorable growing characteristics and its high nutritional value, but it is susceptible to the variety of fungal pathogens that can limit its yield and shorten the plants lifespan. Rhizoctonia spp. represent a very important genus of phytopathogenic fungi that inhabit the soil and can infect a vast verity of cultivated plants (Vojvodić, 2021). Sampling of red clover plant F2 with
symptoms of stunting and necrosis of leaves and stems was done on 05.11.2020. on red clover monoculture plot in Globoder, Serbia (43°34'56.9"N 21°12'08.2"E). Plant F2 root surface was dark colored with sporadical cracks in the root epidermis. The root cross sections showed dark brown necrotic tissue of central cylinder, which was used for pathogen isolation. Obtained isolate had an average growth of 75,67 mm after seven days at 25° C and was designated F2B.
After acquiring a hyphal tip fungal colony macromorphological and micromorphological features were examined on PDA medium. The culture had a woolly structure, mycelium color was paled beige with shades of light brown, sclerotia structures were not present. Microscope examination showed septate hyphae (average width 9,3 µm) with characteristic 90° branching, constriction at the branching point and a septa in the immediate vicinity (Picture 1.). Obtained
morphological features were in accordance with the features of the Rhizoctonia genus. Pathogenicity of the isolate was confirmed on red clover plants and detached plant parts by three pathogenicity tests. The first test consisted of detached red clover leaves inoculation and measuring of lesion diameter (average 6.23 mm), the second was inoculation of red clover stem fragments (40 mm) with measuring of necrotic stem part length (average 27,65 mm) after seven
days of incubation at 25° C. The third pathogenisity test consisted of inoculation of 150 days old red clover plants in semi controlled environment (Yli-Mattila et al., 2008). Inoculation spot was on the main clover roots, about 20 mm from the plant crown and scoring was done 60 days after. Leaves and stems symptoms were visible on seven plants, but the root symptoms were present in all 12 treatment plants, while control plants remained symptomless. Average width
of the root necrotic tissue was 2,36 mm and length was 24,28 mm. Species level identification was done by isolating genomic DNA followed by PCR amplification of the internal transcribed spacer (ITS) using ITS1/ITS4 primer pair. BLAST analysis of the nucleotide sequence revealed that isolate F2B was identical with several reference sequences of binucleate Rhizoctonia AGA deposited in NCBI GenBank. Based on morphological, pathogenic and molecular features
of the isolate F2B, the pathogen of red clover that caused rot root was identified as binucleate Rhizoctonia AG-A.
Variability and heritability of dry matter yield components of perennial ryegrass (Lolium perenne L.) genotypes
(2025-09-01) Sokolović, Dejan; Babić, Snežana; Radović, Jasmina; Lugić, Zoran; Prijović, Mladen
Perennial ryegrass (Lolium perenne L.) is one of the most important perennial forage grasses in the northern climate. It is characterized with high quality, digestibility and dry matter yield. The aim of breeding of this species is obtaining cultivars with high stabile yield, quality and field resistance, with different time of maturity. Wild populations are good initial breeding material, which have high variability and adaptability what is important prerequisite for selection of superior genotypes. The main disadvantage of that material is lack of uniformity, which is hard to achieve before cultivar registration. In the directed breeding process, despite decreasing of some traits total variability, some desirable level of heterogeneity remain what gives the genotype better adaptability. Nevertheless, improvement of some important agronomic traits interact with value of other ones.Investigations of variability parameters (variance components and coefficients of variation) and heritability of perennial ryegrass genotypes for agronomic most important traits are shown here, as well as their correlations. Prosperous perennial ryegrass genotypes originated from wild populations were investigated in space-plant nursery (60x60cm). Time of tillering, plant height, sward diameter, number of tillers per plant, dry matter yield (DMY) and crude protein content were investigated. For plant height and DMY the highest differences between genotypic and phenotypic variance components were determined and the lowest heritability (16.4% and 25.4%), respectively. Highest heritability is determined for time of tillering (83.26%) and sward diameter (79.87%). According the data, breeding of genotypes for improvement of these traits could provide effective results in short period. Also high heritability was detected for number of tillers per plant and crude protein content.
THE INFLUENCE OF WEATHER ON THE QUALITY OF HONEY IN RASINA REGION FOR THE PERIOD OF 2019-2024
(Faculty of Agriculture, University of East Sarajevo, Republic of Srpska, Bosnia and Herzegovina, 2025-10-02) Jevtić, Goran; Babić, Snežana; Andjelković, Snežana; Lazarević, Đorđe; Petrović, Mirjana; Zornic, Vladimir; Matović, K.
The paper monitored the influence of weather conditions (temperature, air humidity, sunshine and rainfall) on the quality of acacia honey, meadow honey and honeydew in the period 2019- 2024. from the territory of the Rasin district in Serbia. Weather conditions were monitored both throughout the year and for 30 days during the acacia flowering. The parameters of honey quality were determined according to the methods of the Rulebook on the quality of honey and other bee products (2015). According to this rulebook, the following parameters of honey quality were determined: the amount of water, free acids, reducing sugars, sucrose, substances insoluble in water, hydroxymethylfurfural (HMF), diastase activity and electrical conductivity. When it comes to the weather in this period, two years stand out. One of these was 2024, dry and warm with high temperatures, low humidity and little precipitation. The other is 2023,
which was warm and humid, when a high average temperature was measured, but also the highest amount of precipitation and the highest air humidity. After the weather conditions were followed 30 days since the flowering of acacia, we concluded that the year 2022 was the warmest, and that the other parameters were the highest in 2023. The weather conditions, in all three analyzed types of honey, had the greatest influence on the amount of water and the amount
of free acids. In 2023, all three types of honey had the most water and the most free acids. Acacia honey had slightly more water in 2021 and less sucrose in 2023. Honeydew has an increased content of free acids and their presence in honey does not depend much on weather conditions.