Browsing by Author "Babić, Snežana"

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An overview of the flora of the special nature reserve "Osredak" a decade upon the completion of the conservation study
(Centre for Evaluation in Education and Science (CEON/CEES), 2022-01-25) Petrović, Mirjana; Prijović, Mladen; Živković-Antić, Ivana; Stojanović, Verica; Vymyslický, Tomaš; Stepić, Marija; Babić, Snežana; Sokolović, Dejan
Since 2020, the "Osredak" Special Nature Reserve has been a protected natural area of the Republic of Serbia, of regional, i.e. great importance. It is situated in central Serbia, along both banks of the West Morava. The area was designated as protected in order to conserve the bog habitats of the Morava River valley, as well as old bayous, ponds and lakes, which were created by decades of gravel extraction. The public-utility company "Kruševac" manages this area, and at its request, cooperation was established with the Istitute for fodder crops Kruševac in order to update the Reserve's flora inventory, with an emphasis on protected plant species, but also on invasive alien species. A list of all plant taxa that have been recorded in the Reserve so far (169 species) and specific solutions that might contribute to the conservation and improvement of natural values of the "Osredak" special nature reserve have also been included in this paper.
Analysis of the relationship of the most important traits in meadow fescue
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2021-10-07) Babić, Snežana; Sokolović, Dejan; Andjelković, Snežana; Petrović, Mirjana; Zornic, Vladimir; Prijović, Mladen; Bekčić, Filip
Meadow fescue (Festuca pratensis Huds.) is one of the most important perennial grasses foranimal feed production on grasslands. It is characterized by high and stabile yield potential andgood biomass quality. The aim of this study was to determine genetic and phenotypic correlation coefficients of seven most important traits of two synthetic cultivars (Kruševački 21 and Pradel). Individual plants for hybridization, in order to obtain progeny by the method of Comstock and Robinson, were selected completely randomly. In order to examine the obtained progeny, the study was performed in an experimental field of the Institute for Forage Crops, in two replications. The experiment was set up according to the Nested Design I, with sets within the replications. In Kruševački 21, 60 full-sib progeny were studied in two sets, while in the Pradel a total of 39 full-sib progeny were studied. Within each full-sib progeny, 60 plants were analyzed. Statistically and high statistically relation was obtained between some traits and presented as genetic (rg) and phenotypic (rf) correlation coefficients. In cultivar K-21 very high statistically significant genetic correlation coefficients were determined between heading date and leaf length (rg=0.873**), plant height and number of generative tillers per plant (rg=0.893**) and dry matter yield in the first cut and annual dry matter yield (rg=0.988**). In cultivar Pradel very high statistically significant relationship was obtained between heading date and plant height (rg=0.978**), heading date and number of vegetative tillers per plant (rg=0.926**) and annual dry matter yield with one side, and plant height (rg=0.912**) and dry matter yield in the first cut (rg=0.978**), on the other side.
ANTIFUNGAL ACTIVITY OF RHIZOBIA AGAINST RED CLOVER PATHOGENS FROM GENUS FUSARIUM
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia University of Belgrade, Faculty of Agriculture, Serbia Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB) Italy, 2023-10-05) Bekčić, Filip; Duduk, Natasa; Lugić, Zoran; Radović, Jasmina; Babić, Snežana; Stepić, Marija; Andjelković, Snežana
Red clover (Trifolium pratense L.) is an important forage legume that can be found in nature or as cultivated crop in monoculture or in grass-legume mixtures. The great importance of red clover is the fact that it lives in symbiosis with Rhizobium leguminosarum bv. trifoli. In addition to promoting plant growth, productivity and biomass, the bacteria also have an important role in disease control. Apart from useful microorganisms, many soil pathogenic microorganisms inhabit red clover roots and under favorable conditions they cause infections and diseases of the cultivated plants. On the infected red clover plants Fusarium spp. can cause several symptoms: vascular wilt on older plants, dumping of seedlings, root rot, rot of root base and crown rot. In the present study, the antagonistic potential was evaluated for two bacteria isolates preliminarily determined as R. leguminosarum bv. trifoli (CD1 and CD6) against three isolates Fusarium spp. (G1, G2 and G3) who were obtained from the roots of symptomatic red clover plants. The research was performed in in vitro agar plate (PDA medium) by methods of dual culture (“bacterial ring”). The comparison of growth assessment of fungi after they were grown together with rhizobia in a single plate and growth of fungi in individual plates was performed. Both rhizobia isolates showed significant antifungal activity against all the tested phytopathogenic fungi. Growth inhibition of G1, G2 and G3 was evaluated (%) using both rhizobia isolates, CD1 varied from 30 to 43 % and CD6 from 14 to 32 %.
Binucleate Rhizoctonia AG-A pathogen of red clover in Serbia
(2025-09-01) Bekčić, Filip; Milenković, Jasmina; Babić, Snežana; Stepić, Marija; Andjelković, Snežana; Duduk, N.; Vico, I.
Red clover (Trifolium pratense L.) in Serbia has a long history of cultivating due to its favorable growing characteristics and its high nutritional value, but it is susceptible to the variety of fungal pathogens that can limit its yield and shorten the plants lifespan. Rhizoctonia spp. represent a very important genus of phytopathogenic fungi that inhabit the soil and can infect a vast verity of cultivated plants (Vojvodić, 2021). Sampling of red clover plant F2 with symptoms of stunting and necrosis of leaves and stems was done on 05.11.2020. on red clover monoculture plot in Globoder, Serbia (43°34'56.9"N 21°12'08.2"E). Plant F2 root surface was dark colored with sporadical cracks in the root epidermis. The root cross sections showed dark brown necrotic tissue of central cylinder, which was used for pathogen isolation. Obtained isolate had an average growth of 75,67 mm after seven days at 25° C and was designated F2B. After acquiring a hyphal tip fungal colony macromorphological and micromorphological features were examined on PDA medium. The culture had a woolly structure, mycelium color was paled beige with shades of light brown, sclerotia structures were not present. Microscope examination showed septate hyphae (average width 9,3 µm) with characteristic 90° branching, constriction at the branching point and a septa in the immediate vicinity (Picture 1.). Obtained morphological features were in accordance with the features of the Rhizoctonia genus. Pathogenicity of the isolate was confirmed on red clover plants and detached plant parts by three pathogenicity tests. The first test consisted of detached red clover leaves inoculation and measuring of lesion diameter (average 6.23 mm), the second was inoculation of red clover stem fragments (40 mm) with measuring of necrotic stem part length (average 27,65 mm) after seven days of incubation at 25° C. The third pathogenisity test consisted of inoculation of 150 days old red clover plants in semi controlled environment (Yli-Mattila et al., 2008). Inoculation spot was on the main clover roots, about 20 mm from the plant crown and scoring was done 60 days after. Leaves and stems symptoms were visible on seven plants, but the root symptoms were present in all 12 treatment plants, while control plants remained symptomless. Average width of the root necrotic tissue was 2,36 mm and length was 24,28 mm. Species level identification was done by isolating genomic DNA followed by PCR amplification of the internal transcribed spacer (ITS) using ITS1/ITS4 primer pair. BLAST analysis of the nucleotide sequence revealed that isolate F2B was identical with several reference sequences of binucleate Rhizoctonia AGA deposited in NCBI GenBank. Based on morphological, pathogenic and molecular features of the isolate F2B, the pathogen of red clover that caused rot root was identified as binucleate Rhizoctonia AG-A.
Biomass and Protein Yields of Field Peas and Oats Intercrop Affected by Sowing Norms and Nitrogen Fertilizer at Two Different Stages of Growth
(MDPI AG, 2021-09-10) Krga; Simić, Aleksandar; Željko, Dželetović; Babić, Snežana; Katanski, Snežana; Roljević Nikolić, Svetlana; Damnjanović, Jelena
In limited growing conditions, intercropped field peas and oats can represent a significant source of forage rich in protein. If applied correctly, factors such as nitrogen fertilizer, the mowing phase, and sowing norms can significantly increase the productivity of these mixtures. Field trials were conducted to examine their productivity under different nitrogen levels (0, 40, 80 kg ha−1), different sowing norms/mixtures (field peas: oats—100:15%; 100:30%), and two stages of growth (full flowering, full pod formation). Nitrogen fertilizer and different sowing norms had a significant effect on the biomass, hay, and crude protein yields. On average, the highest hay yields were achieved with 80 kg ha−1 N (4.96 t ha−1), followed by 40 kg ha−1 N (4.27 t ha−1). The highest protein yields were achieved with 40 kg ha−1 N (CP—704.1 kg ha−1), followed by 80 kg ha−1 N (CP—637.6 kg ha−1). Sowing norm 100:30% achieved higher hay yields: 100:30%—4.82 t ha−1; 100:15%—4.44 t ha−1, while 100:15% achieved higher crude protein yields: 100:15%—730.4 kg ha−1; 100:30%—692.7 kg ha−1 on average. The costs were not significantly increased with the nitrogen fertilizer, but the net profits were increased by as much as 163%, depending on the nitrogen level and the mixture. Nitrogen fertilizer also achieves higher economic efficiency for the mixture 100:15% compared to the 100:30% mixture. Mixtures of field peas and oats outperform single-grown crops and provide cost-effective feed for a short time. Using optimal seed ratios and nitrogen fertilizer can significantly increase the productivity and profitability of the feed with minimal impact on the overall production costs.
Biomass and Protein Yields of Field Peas and Oats Intercrop Affected by Sowing Norms and Nitrogen Fertilizer at Two Different Stages of Growth
(MDPI AG, 2021-09-10) Krga; Simić; Dželetović; Babić, Snežana; Katanski; Nikolić; Damnjanović
In limited growing conditions, intercropped field peas and oats can represent a significant source of forage rich in protein. If applied correctly, factors such as nitrogen fertilizer, the mowing phase, and sowing norms can significantly increase the productivity of these mixtures. Field trials were conducted to examine their productivity under different nitrogen levels (0, 40, 80 kg ha−1), different sowing norms/mixtures (field peas: oats—100:15%; 100:30%), and two stages of growth (full flowering, full pod formation). Nitrogen fertilizer and different sowing norms had a significant effect on the biomass, hay, and crude protein yields. On average, the highest hay yields were achieved with 80 kg ha−1 N (4.96 t ha−1), followed by 40 kg ha−1 N (4.27 t ha−1). The highest protein yields were achieved with 40 kg ha−1 N (CP—704.1 kg ha−1), followed by 80 kg ha−1 N (CP—637.6 kg ha−1). Sowing norm 100:30% achieved higher hay yields: 100:30%—4.82 t ha−1; 100:15%—4.44 t ha−1, while 100:15% achieved higher crude protein yields: 100:15%—730.4 kg ha−1; 100:30%—692.7 kg ha−1 on average. The costs were not significantly increased with the nitrogen fertilizer, but the net profits were increased by as much as 163%, depending on the nitrogen level and the mixture. Nitrogen fertilizer also achieves higher economic efficiency for the mixture 100:15% compared to the 100:30% mixture. Mixtures of field peas and oats outperform single-grown crops and provide cost-effective feed for a short time. Using optimal seed ratios and nitrogen fertilizer can significantly increase the productivity and profitability of the feed with minimal impact on the overall production costs.
Botanical composition and biomass quality in natural grasslands of southeast part of Serbia
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia University of Belgrade, Faculty of Agriculture, Serbia Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB) Italy, 2022-10-06) Zornic, Vladimir; Petrović, Mirjana; Babić, Snežana; Sokolović, Dejan; Prijović, Mladen; Tomić, Dalibor; Lazarević, Đorđe
Ca:P ratio and content of Ca and P in some annual legumes – oat mixtures depending on seeding rate and stage of growth.
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia University of Belgrade, Faculty of Agriculture, Serbia Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB) Italy, 2020-10-08) Marković, Jordan; Vasić, T.; Andjelković, Snežana; Petrović, Mirjana; Bekčić, Filip; Lazarević, Đorđe; Babić, Snežana
Calcium and phosphorus are particularly important for animal health. The dietary levels of Ca and P should be balanced to increase their availability and utilization. The research was conducted at Institute for forage crops Kruševac in 2015-2016 to determine the effects of seeding rates in mixtures of pea : oat and common vetch : oat and cutting stages on the Ca and P content, as well as Ca : P ratio. The experiment was performed using five different mixture rates of pea and oat crops, five different mixture rates of common vetch and oat crops and three different cutting stages (beginning of pea and common vetch flowering – 10% of flowering, forming the first pods on 2/3 pea and common vetch plants and forming green seeds in 2/3 pods). Results of investigation showed that Ca content was the highest at the forming the first pods on 2/3 pea and common vetch plants, while phosphorus content increased from 2.70 to 3.40 g kg-1 with common vetch growth and development and from 2.57 to 3.10 g kg-1 with pea growth and development. Pure sown common vetch and pea were characterized by the highest average Ca and P content. Ca : P ratio decreased with plant growth and development in pea : oat mixtures, as well as in common vetch : oat mixtures. The highest Ca : P ratio was recorded in pure sown common vetch and pure sown pea, whereas the lowest Ca : P ratio was recorded in pure sown oat.
EFFECT OF AZOTOBACTER SPP. ON INITIAL GROWTH OF GRASSES
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia University of Belgrade, Faculty of Agriculture, Serbia Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB) Italy, 2023-10-05) Andjelković, Snežana; Sokolović, Dejan; Lugić, Zoran; Jevtić, Goran; Radović, Jasmina; Milenković, Jasmina; Babić, Snežana
The composition of the microbial community in rhizosphere can affect plant growth, nutrients uptake, and stress tolerance. In rhizosphere bacteria belonging to the genus Azotobacter there are free nitrogen-fixers promoting growth and health of plants. The aim of this research was to investigate the effect of different native isolates (SA74, SB94, MA7 and their combination) Azotobacter spp. on initial growth of plants tall fescue (Festuca arundinacea Scherb) and meadow fescue (Festuca pratensis Huds). The experiment was carried out in semi-controlled conditions. For this study, seeds of tall fescue cultivar Kruševački 20 (K-20) and meadow fescue cultivar Kruševački 21 (K-21) were planted in pots filled with alluvial soil with slightly acidic reaction. The height, green mass per plant, root length and root weight per plant were determined. Four variants of microbial inoculation were compared with the growth of non-inoculated control. The significance of difference between examined treatments was determined by Fisher’s LSD test (p<0.05). At tall fescue, applied isolate SB94 in all investigated parameters had a negative effect. The two other isolates as well as combination of three isolates had a positive effect on initial growth of plants. Only in plant height, the application of a combination of isolates did not have positive result compared to the control. At meadow fescue, inoculation with combined isolates positively influenced plant height andgreen mass per plant. Root length was significantly increased only in treatment with isolate MA7. The influence of inoculation on root weight per plant was not noted.
ENVIRONMENTAL ASSESSMENT OF SOME HEAVY METALS IN SELECTED MEDICINAL PLANT SPECIES ALONG A BUSY ROAD IN VRNJAČKA BANJA, SERBIA
(Asociatia Carpatica de Mediu si Stiintele Pamantului, 2022-08-31) BABIĆ; MITRA; DAS MOHAPATRA; PAPOVIĆ; Milenković, Jasmina; Jevtić, Goran; Babić, Snežana; Andjelković, Snežana
This study reported the concentrations of Pb, Cd, Cr, Mn in parts of four medicinal plant species (Cichorium intybus L., Mentha×piperita L., Plantago lanceolata L., Verbascum densiflorum Bertol.) collected from two sites, one few meters away and the other 500 m away from a busy road in Vrnjačka Banja, Serbia. Heavy metals were determined by the atomic absorption method using atomic absorption spectrophotometer. Similar content of tested metals in the soil of the tested locations was recorded, only there was more manganese in the soil closer to the road. The highest concentrations of heavy metals were found in the roots of plants closer to the road. The leaves and stem contain smaller quantities of these metals than root, but there are differences between the studied plant species. In conclusion, all examined metals were present in higher concentrations in plants at the location closest to the road than at the location further away from the road.
Examination of Microbiological Properties of Agricultural Soils on Area the Municipality of Lučani
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2025-10-02) Jevtić, Goran; Andjelković, Snežana; Babić, Snežana; Lazarević, Đorđe; Racić, Nedeljko; Mitra, D.; Zornic, Vladimir
Expected genetic gain of the most important traits in the breeding process of the meadow fescue.
(2025-09-01) Babić, Snežana; Sokolović, Dejan; Prijović, Mladen; Andjelković, Snežana; Lazarević, Đorđe; Zornic, Vladimir; Radović, Jasmina
Meadow fescue (Festuca pratensis Huds.) is an important perennial forage grass for animal feed production on grasslands and pastures, with high yield potential and good-quality biomass. During the breeding process, it is often necessary to predict, based on the results of the initial selection cycles, how much a certain trait can be improved. To that end, it is necessary to determine the expected genetic gain (ΔGe), a parameter that tells us how much a trait can be improved by breeding it alone in the following period. Individual plants for hybridization, to obtain progeny by the method of Comstock and Robinson, were selected completely randomly. To examine the obtained progeny, the study was performed in an experimental field of the Institute for Forage Crops, in two replications. The experiment was set up according to the Nested Design I, with sets within the replications. In cv. Kruševački 21 (K-21), 60 full-sib progeny were studied in two sets, while in the cv. Pradel a total of 39 full-sib progeny were studied. Within each full-sib progeny, 60 plants were analyzed. In both cultivars, the highest expected genetic gain was determined for the number of generative and vegetative tillers per plant and dry matter yield per plant. The least success can be achieved by improving the heading date and crude protein content in the 1st cut in cv. K-21, and in cv. Pradel for traits leaf length and crude protein content in the 1st cut. By breeding individual traits, based on the obtained results, a large genetic gain is expected in many studied traits. Considering the existence of correlations between the most important agronomic traits, it is observed that often, by breeding one trait, a negative response is encountered in another trait. For this reason, the expected indirect genetic gain from selection was calculated, i.e., the effect of breeding one trait on other ordered traits. In the cv. K-21, achieving the expected genetic gain in plant height, would lead to a decrease in dry matter yield and leaf length. By creating later cultivars, the result would be a cultivar with a smaller number of generative tillers and a lower protein content in the first cut. Successful breeding for increased crude protein content in the first cut of 9.9 gkg-1 would result in a significant reduction in dry matter yield, followed by a reduction in the number of vegetative and generative tillers, but also earlier maturity. In cv. Pradel, a positive effect of the selection of individual traits on other studied traits was recorded for most of the traits. The only realization of the expected genetic gain in the increase in leaf length would result in a decrease in the number of vegetative tillers per plant.
Fertility and chemical composition of forest soils covered with Allium ursinum L. In Serbia
(Parlar Scientific Publications, 2022-04) Dželetović, Ž.,; Simić, A.; Marković, Jordan; Andrejić, G.; Denader, T.; Babić, Snežana
A. ursinum is a wild growing species, which is often found on various localities throughout Serbia, primarily within forest and occasionally on meadow. Fresh picked leaves and bulbs are used for preparing a salad or meals. For this research we have collected and analyzed soil and plant material from 12 different locations in Serbia. The analyses of basic fertility of soils have shown that A. ursinum grows mainly on acidic and soils containing high percentage of humus, with high total nitrogen content, low to medium supplies in available phosphorus and high supplies in available potassium. In those soils there is a very strong correlation between the total N content and total organic C (0.931). There is a relatively wide range of concentrations of overall Cd, Co, Cr, Cu, Ni, Pb and Zn in soils on which A. ursinum grows. The translocation factor for A. ursinum is TF ≥1 has been calculated for Cd, Pb and Zn.
Fertility and chemical composition of forest soils covered with Allium ursinum L. In Serbia
(2022-04) Dželetović, S. Ž.; Simić, A.; Marković, Jordan; Andrejić, G.; Denader, T.; Babić, Snežana
Fiziološki i biohemijski odgovori engleskog ljulja (Lolium perenne L.) na vodni deficit: Procena potencijala za oplemenjivanje novih sorti otpornih na sušu.
(Институт за крмно биље, Крушевац Друштво за крмно биље Републике Србије, Крушевац, 2024-09-26) Prijović, Mladen; Sokolović, Dejan; Babić, Snežana; Babić, Snežana; Zornic, Vladimir; Racić, Nedeljko; Lazarević, Đorđe; Sabovljević, Aneta
У светлу глобалних климатских промена, обезбеђивање довољне количине хране постаје све већи изазов. Узгајање високоприносних и на стрес отпорних сорти крмног биља и житарица кључ је успешне и одрживе сточарске производње. Енглески љуљ (Lolium perenne L.), због својих бројних корисних особина, представља једну од најважнијих вишегодишњих крмних трава умереног климата, чинећи основу многих ливада и пашњака. Како се ови системи не заливају, енглески љуљ је све више изложен дуготрајним сушама током године, посебно у летњим месецима. Стога постоји хитна потреба за побољшањем његове толеранције на сушу и одрживости у умерено- континенталном климату, нарочито на травњацима са све чешћим дефицитом воде (Bothe, 2018). Једна од стратегија је унапређење физиолошких и биохемијских особина код нових сорти кроз различите процесе оплемењивања, без значајног нарушавања нутритивне вредности и прилагодљивости испаши. Да би се то постигло, потребно је прво детаљно испитати све факторе адаптације и аклиматизације на сушу, који би се могли користити као критеријуми за унапређење толеранције на сушу код будућег оплемењивачког материјала а затим идентификовати и њихову молекуларну основу, што је циљ овог истраживања. Испитивање варијабилности енглеског љуља на водни дефицит спроведено је на одабраним генотиповима из атлантског (сорта Shandon) и умерено-континенталног климата (сорта К-11 и експериментална оплемењивачка популација) у фази вегетативног раста.
Forage quality of different Festulolium cultivars
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2022-10-06) Babić, Snežana; Sokolović, Dejan; Andjelković, Snežana; Petrović, Mirjana; Jevtić, Goran; Prijović, Mladen; Racić, Nedeljko
Hybrids created by crossing between genera Festuca and Lolium species are Festulolium.Crossing species of these genera aim to combine their positive characteristics, such as Festuca tolerance to abiotic stresses and edaphic and climatic conditions with the high quality and digestibility of Lolium perenne and Lolium multiflorum species. Forage quality of 15 different Lolioid Festulolium cultivars and six cultivars of Festuca and Lolium pure species were investigated in a moderate continental climate in Kruševac, Serbia. Plots in the trial (7,5 m2 ) were completely randomized, each in three replications. In the year of establishment, the plots were cut without weighing and taking samples. In the next two years, two cuts were taken. The first cut was done at the beginning of the heading (the first half of May) and the second in the first half of July. On dry samples, from two cuts, the content of crude protein, crude cellulose, crude fat, ash, ADF, NDF and ADL was determined by standard laboratory methods. Results were presented as two-year average values. The best dry matter quality of Festulolium cultivars was determined for cultivars AberNiche and Felopa. The highest crude protein content in the first cut was determined for AberNiche (187.1 gkg-1 ) and Felopa (169.6 gkg-1). Also, the same cultivars noted the lowest values for ADF (296.7 and 303.5 gkg-1 ) and for AberNiche was determined the lowest ADL contet (35.4 gkg-1 ) in the first cut. These cultivars are in the group of cultivars with the lowest NDF value. Based on the obtained results, it can be concluded that the analyzed Festulolium cultivars have a higher crude protein content compared to the cultivars of Festuca pure species, but lower than Lolium species.
Genotype by environment interactions and phenotypic traits stability of the EUCLEG faba bean collection
(Frontiers Media SA, 2025-01-29) Sokolović, Dejan; Babić, Snežana; Petrović, Mirjana; Solís, D.; Cougnon, M.; Gutierrez, N.; Pärssinen, P.; Reheul, D.; Radović, Jasmina; Torres, A. M.
Faba bean (Vicia faba L.) is an important pulse crop traditionally used for human nutrition and animal feeding. With a high protein content ranging from 24% to 35% of seed dry matter, considerable amounts of globulins, essential amino acids and minerals, faba bean is today an important source meeting the growing global demand for nutritious food. The objective of study was to investigate the variability of nine phenological, phenotypical and yield related traits in 220 faba bean accessions in multi-location trials across four representative European regions. Nine field trials were carried out from 2018 till 2020 in four representative European locations (Spain, Finland, Belgium and Serbia) using an augmented p-rep design containing 20 replicated checks. Significant differences among genotypes and environments were detected, being the genotype x environment interaction (GEI) the major source of variation in five of the nine evaluated traits. The “which-won-where” analyses identified two mega-environment namely South European mega environment (SE-ME) and North European mega environment (NE-ME), while the best performing and most stable genotypes according to the nine traits were identified using “means vs stability” analyses. According to the highest trait value in each mega environment several winning genotypes were identified showing better performances than some commercial varieties (controls) or checks. Our results suggest that the geographical locations falling into each mega-environment can be used as faba bean test locations. The genotype ranking for the multi-trait stability index (MTSI) revealed that the most stable and best ranking genotypes in SE-ME are G018, G086, G081, G170 and G015 while in the north mega-environment are G091, G171, G177 (Merkur), G029 and G027. Hierarchical cluster analysis and principal component analyses showed a clear correlation between the traits analysed and the botanical type. These findings indicate that botanical type is one of the most significant factors affecting development in any environment, and it must be taken into account in faba bean breeding activities. The information derived from this study provides a chance for breeding new resilient faba bean cultivars adapted to different agroecological European regions, a critical point for addressing Europe’s reliance on protein imports and enhancing sustainable agriculture practices.
Heritability and variability of the most important traits of meadow fescue synthetic cultivars
(Agricultural Research Ltd. Troubsko, Czech Republic, 2023-09-10) Babić, Snežana; Radović, Jasmina; Andjelković, Snežana; Petrović, Mirjana; Jevtić, Goran; Prijović, Mladen; Sokolović, Dejan
Meadow fescue (Festuca pratensis Huds.) is one of the most important perennial grasses for animal feed production on grasslands. It is characterized by high and stable yield potential and good biomass quality. The aim of this study was to determine the broad and narrow sense heritability and genetic (CVG%) and phenotypic (CVF%) coefficient of variation of the most important traits of two synthetic cultivars (Kruševački 21 and Pradel).
Increasing Drought Tolerance of Forage Perennial Ryegrass by Improvement of Root Characteristics
(Institutul Național de Cercetare Dezvoltare Agricolă Fundulea, 2025) Prijović, Mladen; Sokolović, Dejan; Radović, Jasmina; Petrović, Mirjana; Zornic, Vladimir; Jevtić, Goran; Babić, Snežana
Perennial ryegrass is an essential cool-season forage grass with high-quality biomass, but with pronounced sensitivity to drought. This research was aimed to determine the shoot dry matter and root characteristics of perennial ryegrass populations (cultivar K-11 and breeding population LPb3). 20 randomly chosen individual plants per population were clonally divided into twelve small parts, remets, and transferred into the plastic root-screening tubes for root length and depth density evaluation. Also, shoot dry matter (SDM) was analysed. The experiment was designed as a two-factorial design, with population and watering regime as factors (2x4), in three replications. Analysed populations showed different root and shoot characteristics, especially in treatments with water reduction. Significant differences for most of the analysed traits between treatments were determined. Shoot dry matter was higher by 9% on average in population LPb3 than in K-11. Also, the dry matter yield of the total root dry matter (TRDM) was larger in LPb3. Treatments significantly affected total root dry matter, which dropped with water reduction. Dry matter of roots below 90 cm (RB90) was statistically significantly higher in LPb3 than in K-11. Broad-sense heritability and estimated genetic gain for most analysed traits were higher in population LPb3 than in K-11. The breeding of all investigated traits in this perennial ryegrass material is promising, especially in population LPb3.
Influence of stimulative feeding measures on spring development of bee societies in DB and LR honeycombs
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2022-10-06) Jevtić, Goran; Babić, Snežana; Andjelković, Snežana; Zornic, Vladimir; Lazarević, Đorđe; Matovic, Kazimir; Nedić, N.
In order for the bee colonies to make the most of the main pasture, and primarily black locust, it is very important that they are in good condition. It is necessary for the societies to reach their maximum strength at the beginning of the main pasture, and not for it to serve them for further development. The beekeeper himself plays a crucial role in the accelerated spring development of bee colonies. The aim of this study was to determine the effect of different ways of spring feeding on the strength of bee colonies and the food supply in them. The experiment was performed with companies that were in Db (Dadant-Blatt) and Lr (Langstroth-Ruth) hives. Four groups of companies were tested, and there were five companies in each group. The first group was supplemented with sugar syrup, enriched with vitamin-mineral complex - Foprsapine (10 drops per liter of syrup). The second group of societies was fed with honey dissolved in water (ratio of honey and water 1:1). The third group of the companies was fed with sugar dough (energy cake without additives). The fourth group of companies was supplemented with sugar syrup (the ratio of water and sugar was 1:1). At the beginning of the experiment, the societies in Lr hives had an average of 4.5 frames with bees, and 2.5 frames with a brood. In the Db hives, the societies occupied five frames with bees, and had 2.7 frames with a brood. At the end of the experiment Lr societies had 8.7 frames with bees, and 4.5 frames with brood, while Db societies had nine frames with bees, and 4.7 frames with brood. The experiment was performed at the apiary of the Institute for Forage Crops in Kruševac.