RIFoC
Digitalna archive of the Instiitute for Forage Crops
Primary research results available.
- Institutional digital repository of the Institute for Forage Crops established in December 2023.
- Since April 2024. portal eNauka regularily harvests meta-data from RIFoC repository.
Contents of RIFoC
- Ustanova vodi poreklo od Uglednog dobra i vrta formiranog 2. februara 1884. godine odlukom Podružine kruševačke Srpskog poljoprivrednog društva na Drugom glavnom zboru a “na osnovu tvrdog uverenja da se neposrednim primernim radovima na oglednim dobrima, najjače i najbrže može dejstvovati na unapredjenje poljoprivrede narodne”. U toku svoje istorije, menjani su nazivi i delimično nadležnosti, a od 21. oktobra 1932. godine postaje naučna ustanova spajanjem Agrobotaničkog odseka Poljoprivredne ogledne i kontrolne stanice u Topčideru i Poljoprivredne stanice u Kruševcu
Recent Submissions
Процена ефекта примене ризобијалних сојева на принос луцерке гајене на земљишту са повећаном концентрацијом никла
(Toplička akademija strukovnih studija, 2025-04-23) Andjelković, Snežana; Pešić, M.; Tošić Jojević, S.; Sikirić, B.; Mrvić, V.; Milinković, M.; Stajković Srbinović, O.
Lucerka (Medicago sativa L.) je najznačajnija leguminozna vrsta za stočarstvo, a razlog za to jeste što se ova biljna vrsta odlikuje visokom hranljivom, energetskom i biološkom vrednošću. Zahvaljujući ovim osobimama lucerka se koristi i kao dodatak u ishrani humane populacije, u farmaceutskoj i kozmetičkoj industriji. Lucerka živi u simbioznoj zajednici sa rizobijalnim bakterijama pri čemu u procesu azotofiksacije može da fiksira 43-80 % potrebnog azota. Ova leguminoza može da apsobuje veliku količinu potencijalno toksičnih elemenata, što predstavlja potencijalnu opasnost da oni uđu u lanac ishrane. U sprovedenim istraživanjima ispitivan je uticaj primene sojeva Ensifer meliloti: 218, 224, G-nov, 4193cs, 217k i 252 na prinos suve mase u prvom i drugom otkosu lucerke (sorta K-28) u godini zasnivanja. Eksperiment je postavljen na oglednom polju Instituta za krmno bilje Kruševac po randomiziranom blok sistemu u četiri ponavljanja u proleće 2024. godine na zemljištu sa povećanom koncentracijom nikla. U prvom otkosu masa suve materije kretala se u rasponu od 151,2 do 246,8 g/m2, a u drugom otkosu od 116,6 do 286,4 g/m2. U oba otkosa, na tretmanu na kome je primenjen izolat 252 zabeležena je veća vrednost suve mase u odnosu na konrolu. Takođe, pozitivan efekat je postignut apliciranjem soja 218 u prvom i soja 4193cs u drugom otkosu. Ovo su rezultati prve faze eksperimenta, a u daljim istraživanjima analiziraće se efekat inokulacije ovim sojevima na usvajanje nikla od strane lucerke pošto pri povecanoj koncentraciji u zemljištu, raste sadržaj ovog metala u nadzemnim organima i korenu lucerke, a sa ciljem pronalaženja rešenja za proizvodnju dovoljnih količina kvalitetne i zdravstveno bezbedne stočne hrane.
Antimicrobial resistance in Salmonella: One Health perspective on global food safety challenges
(Elsevier BV, 2025) Kumar, R.; Adeyemi, O. N.; Chattaraj, S.; Alloun, W.; Thamarsha, A.K.A.N.W.M.R.K.; Andjelković, Snežana; Mitra, D.; Gautam, P.
Antimicrobial resistance (AMR) in Salmonella is a major concern in terms of human health, environmental sustainability, and global food security. Salmonella, the world's most prevalent foodborne pathogen, has gradually gained resistance to important drugs, complicating treatment efforts and leading to rising morbidity, mortality, and economic costs. Overuse of antibiotics in human medicine and agriculture, inadequate regulatory compliance, and environmental pollution from agricultural runoff and treated sewage all contribute to the growth and spread of AMR in Salmonella. Health as well as viewpoints on environmental, animal, and human health will be required to address this complicated problem. This includes promoting reasonable antibiotic use, improving global surveillance systems, and researching novel treatments including phage therapy, probiotics, and new antibiotics. Proposals for vaccination and precise food safety standards are critical for limiting Salmonella transmission across the food chain. Despite significant progress, critical research gaps continue, specifically in understanding the molecular basis of resistance and the role of environmental practices. However, contemporary research endeavors are concentrated on identifying and comprehending the prevalence of extensively drug-resistant Salmonella strains, elucidating the bacterium's defense mechanisms against antibiotics, and investigating outbreaks associated with vegetables. Global surveillance, the development of alternative therapies, and the implementation of stricter antibiotic policies are essential strategies in addressing AMR in
Salmonella. Additionally, policy design and implementation, capacity building in low- and middle-income countries, and raising public awareness all necessitate urgent global collaboration among governments, international agencies, non-governmental organizations, and the corporate sector. Stricter regulations on overuse of antibiotics in agriculture is also called for. This review highlights the importance of multi-disciplinary struggles in engaging Salmonella, as integrated resolutions through a One Health approach are crucial for ensuring food safety, preserving public health, and minimizing the worldwide threat of AMR.
Variability of vitamin E content in ZP popcorn hybrids
(Maize Research Institute Zemun Polje, 2025-09-29) Srdić, J.; Vukadinović, J.; Mladenović Drinić, S.; Nikolić, A.; Milenković, Jasmina; Kravić, N.; Anđelković, V.
Vitamin E is a group of eight lipid-soluble antioxidants found in plants, divided into tocopherols and tocotrienols. As an essential nutrient, it supports seed development, stress response, and membrane protection. In humans, it helps protect cell membranes and may reduce the risk of diseases such as Alzheimer's, cancer, neurological disorders and inflammation. Both tocopherols and tocotrienols exist in four isoforms: α (alpha), β (beta), γ (gamma), and δ (delta), classified based on the position of methyl groups on the chromanol ring. This study analyzed α, γ+β, and δ tocopherols (T) and tocotrienols (T3) in five popcorn hybrids grown in Zemun Polje (ZP) and Kruševac (KŠ), Serbia. The content of vitamin E was higher in Zemun Polje, indicating that environmental factors had a significant impact on the content. The average content of α-T was 5.31 μg/g dry weight (DW) at ZP and 4.27 μg/g DW at KŠ, with ranges of 3.99–7.52 μg/g DW and 3.18–5.03 μg/g DW, respectively. The highest α-T content at both locations was recorded in hybrid ZP 6119k. The average content of γ+β-T was 29.63 μg/g DW at ZP and 23.51 μg/g DW at KŠ. δ-T content ranged from 1.69–3.63 μg/g DW at ZP and from 0.89–2.25 μg/g DW at KŠ. The highest α-T3 content at both locations was found in hybrid ZP 614k (5.21 μg/g DW at ZP and 3.62 μg/g DW at KŠ). γ+β-T3 varied from 4.13–7.07 μg/g DW (ZP) and 3.29–6.99 μg/g DW (KŠ). The highest values of δ-T3 were 0.74 μg/g DW in ZP 6153k (ZP) and 0.76 μg/g DW in ZP 6119k and ZP 6170k (KŠ). Popcorn hybrids with higher Vitamin E content, especially ZP 6119k, show potential as functional foods with added health benefits for human consumption.
Seed size and protein content in dry grains of the faba bean (Vicia faba L.) lines originated from Serbian local populations
(LiveSeeding, the Polytechnic University of Coimbra, and supporting organisations and projects, 2025-05-26) Milenković, Jasmina; Petrović, Mirjana; Stepić, Marija; Bekčić, Filip; Andjelković, Snežana; Zornic, Vladimir; Perović, D.
In the past, the faba bean (Vicia faba L.) was an important legume in the Mediterranean diet for both humans and livestock. Today, however, it has largely been forgotten in Serbia. The Serbian Ministry of Agriculture currently recognizes only two commercial varieties of faba bean for animal feed (var. minor). Faba beans intended for human consumption are grown on very small plots of land, primarily for family use. The key factor for the survival of tiny local faba bean populations is the traditional preparation of faba bean tied to Lenten practices observed by Orthodox Christians. During this time, guests are offered a vegan, hummus-style dish made from faba beans. However, this dish is not prepared in all regions of Serbia, or every village. Seeds are infrequently transferred between farmers and are rarely found at the farmer’s markets, so it is feasible to believe that faba bean biodiversity in Serbia is high because maybe every farmer, which produces faba bean, has his own population. The Institute for Forage Crops Kruševac (IKBKS) has launched a research program to collect and evaluate native faba bean populations in Serbia. This collection now has approximately
30 original populations and about 60+ inbreed derived lines. According to preliminary assessments, the grain quality and grain bioactive material content are both a quite high (Milenković et al., 2024, Mitić et al., 2024). In this research, the lines were divided into two groups depending on seed size: major and equine-minor, to evaluate if grain size affects protein content. Because in Serbia the majority of local populations have a major grain size, the lines with equine and minor grain types are joined. The two-year experiment was conducted on the IKBKS plot in two repetitions using a randomized block system. The crude protein content was chemically analyzed using the Kjeldahl method. In this exploratory study, the crude protein content of the dry grain of faba bean lines was investigated in order to choose material for future work on the development of a new high-protein faba bean variety. According to the findings of this study, the protein content of the examined lines ranged between 26-31%, indicating that there is need for future research into the development of high-quality varieties for human or animal consumption. Low-input agriculture is consistent with sustainable farming goals in Serbia, and it can be especially beneficial for smallholder farmers trying to save costs while minimizing their environmental impact. Collecting and cultivating neglected and old species in conventional and organic agriculture can make a significant contribution to healthy food.
Antimicrobial potential of the methanol and water extract of Potentilla argentea L. aerial parts and roots
(Prirodno-matematički fakultet, Univerzitet u Banjoj Luci, 2025-11-13) Duboja Krstić, A.; Novaković, M.; Pešić, S.; Stojanović, D.; Stepić, Marija; Joković, N.; Vitorović, J.
Potentilla argentea L., is traditionally used in Kruševac, Serbia, as an herbal decoction for the treatment of gastrointestinal disorders. This study evaluated and compared the antimicrobial activity of both the plant root and aerial parts (stems, leaves and flowers). P. argentea samples were collected during two different periods: March and May. Samples were dried, ground, and subjected to ultrasonic extraction using methanol (ME) and water (AE). The aqueous extract
of the root was prepared following the traditional method of tea preparation, with modifications involving 24-hour steeping in water prior to filtration, rather than immediate use as in typical preparation. Antimicrobial activity was assessed by determining Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) against seven American Type Culture Collection (ATCC) bacterial strains: Staphylococcus aureus ATCC 6538, Bacillus cereus ATCC 11778, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Salmonella enteritidis ATCC 13076, Enterobacter aerogenes ATCC 13048 and Pseudomonas aeruginosa ATCC 9027. None of the extracts inhibited E.coli, while the strongest antimicrobial activity was observed againts S. enteritidis (0,0098/0,0098 mg/ml). ME from aerial parts exhibited stronger antimicrobial activity compared to root extracts. However, root ME exhibited greater antimicrobial activity against B. cereus compared to aerial part ME, while it showed no antimicrobial activity against E. aerogenes and P. aerogenes. No significant difference was observed between root and aerial parts ME against S. aureus and E. faecalis, though samples harvested in May exhibited greater antimicrobial acitivity in both cases. AE from roots collected in March showed no antimicrobial activity.