Browsing by Author "Bekčić, Filip"

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Analysis of the relationship of the most important traits in meadow fescue
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2021-10-07) Babić, Snežana; Sokolović, Dejan; Andjelković, Snežana; Petrović, Mirjana; Zornic, Vladimir; Prijović, Mladen; Bekčić, Filip
Meadow fescue (Festuca pratensis Huds.) is one of the most important perennial grasses foranimal feed production on grasslands. It is characterized by high and stabile yield potential andgood biomass quality. The aim of this study was to determine genetic and phenotypic correlation coefficients of seven most important traits of two synthetic cultivars (Kruševački 21 and Pradel). Individual plants for hybridization, in order to obtain progeny by the method of Comstock and Robinson, were selected completely randomly. In order to examine the obtained progeny, the study was performed in an experimental field of the Institute for Forage Crops, in two replications. The experiment was set up according to the Nested Design I, with sets within the replications. In Kruševački 21, 60 full-sib progeny were studied in two sets, while in the Pradel a total of 39 full-sib progeny were studied. Within each full-sib progeny, 60 plants were analyzed. Statistically and high statistically relation was obtained between some traits and presented as genetic (rg) and phenotypic (rf) correlation coefficients. In cultivar K-21 very high statistically significant genetic correlation coefficients were determined between heading date and leaf length (rg=0.873**), plant height and number of generative tillers per plant (rg=0.893**) and dry matter yield in the first cut and annual dry matter yield (rg=0.988**). In cultivar Pradel very high statistically significant relationship was obtained between heading date and plant height (rg=0.978**), heading date and number of vegetative tillers per plant (rg=0.926**) and annual dry matter yield with one side, and plant height (rg=0.912**) and dry matter yield in the first cut (rg=0.978**), on the other side.
ANTIFUNGAL ACTIVITY OF RHIZOBIA AGAINST RED CLOVER PATHOGENS FROM GENUS FUSARIUM
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia University of Belgrade, Faculty of Agriculture, Serbia Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB) Italy, 2023-10-05) Bekčić, Filip; Duduk, Natasa; Lugić, Zoran; Radović, Jasmina; Babić, Snežana; Stepić, Marija; Andjelković, Snežana
Red clover (Trifolium pratense L.) is an important forage legume that can be found in nature or as cultivated crop in monoculture or in grass-legume mixtures. The great importance of red clover is the fact that it lives in symbiosis with Rhizobium leguminosarum bv. trifoli. In addition to promoting plant growth, productivity and biomass, the bacteria also have an important role in disease control. Apart from useful microorganisms, many soil pathogenic microorganisms inhabit red clover roots and under favorable conditions they cause infections and diseases of the cultivated plants. On the infected red clover plants Fusarium spp. can cause several symptoms: vascular wilt on older plants, dumping of seedlings, root rot, rot of root base and crown rot. In the present study, the antagonistic potential was evaluated for two bacteria isolates preliminarily determined as R. leguminosarum bv. trifoli (CD1 and CD6) against three isolates Fusarium spp. (G1, G2 and G3) who were obtained from the roots of symptomatic red clover plants. The research was performed in in vitro agar plate (PDA medium) by methods of dual culture (“bacterial ring”). The comparison of growth assessment of fungi after they were grown together with rhizobia in a single plate and growth of fungi in individual plates was performed. Both rhizobia isolates showed significant antifungal activity against all the tested phytopathogenic fungi. Growth inhibition of G1, G2 and G3 was evaluated (%) using both rhizobia isolates, CD1 varied from 30 to 43 % and CD6 from 14 to 32 %.
ANTIFUNGALNA AKTIVNOST ETARSKOG ULJA SATUREJA MONTANA L. PREMA ALTERNARIA SP., PATOGENA STEPSKOG BOŽURA
(Društvo za zaštitu bilja Srbije, Nemanjina 6, 11080 Beograd, 2023-11-27) Mikić, Sara; Stević, Tatijana; Mrđan, Snežana; Vučković, Nina; Bekčić, Filip; Prijić, Željana; Marković, Tatijana
Rtanjski čaj, planinski čubar ili vrijesak (Satureja montana L.) je višegodišnja žbunasta biljka iz familije Lamiaceae, koja vodi poreklo iz umereno toplih područja Evrope, Mediterana i Afrike. U narodnoj medicini se koristi kao čaj za lečenje bolesti respiratornog, digestivnog i urinarnog sistema. Za spoljnu upotrebu se koristi prilikom upala kože i sluzokože, a kao začin i aromatični dodatak jelima u kulinarstvu. U industrijskoj preradi se koristi za dobijanje etarskog ulja. Etarsko ulje se dobija iz nadzemnog dela biljke. Hemijske komponente ulja su karvakrol, timol, β-kariofilen, γ-terpen, ρ-cimen, linalol i druge. U industriji parfema se kombinuje sa drugim uljima ili koristi samostalno. Cilj istraživanja bio je da se ispita antifungalna aktivnost tri hemotipa etarskog ulja Satureja montana L. prema Alternaria sp. izolovanu iz stabla stepskog božura (Paeonia tenuifolia L.).
GENETIČKI ODGOVOR LUCERKE NA TOKSIČNO DELOVANJE JONA ALUMINIJUMA NA KISELOJ HIDROPONSKOJ KULTURI
(Društvo genetičara Srbije, Beograd, 2023-10-16) Stepić, Marija; Bekčić, Filip; Prijović, Mladen; Radović, Jasmina; Zdravković, Jasmina
Toksičan efekat aluminijuma veoma je važan faktor koji ograničava rast i razviće korena lucerke (Medicago sativa L.) na kiselim zemljištima što dovodi do smanjenja prinosa. Cilj eksperimenta bio je da se utvrdi uticaj aluminijuma na: dužinu korena, svežu nadzemnu masu, svežu masu korena, broj bočnih korenova, dužinu bočnih korenova i na rastojanje do prvog bočnog grananja na korenu kod klijanaca tri sorte lucerke (Zuzana, K-28, O-66) u hidroponskoj kulturi u kontrolisanim uslovima. Korišćen je Hoaglandov rastvor ½ jačine pH vrednosti 4.5, za tretman je korišćen aluminijum u formi aluminijum-hlorida (koncentracije 100 µM) i za analize je korišćeno 40 klijanaca po sorti.
NUTRITIVE VALUE OF ITALIAN RYEGRASS-RED CLOVER MIXTURES DEPENDING ON THE SEEDING RATE IN THE MIXTURES
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia University of Belgrade, Faculty of Agriculture, Serbia Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB) Italy, 2023-10-05) Marković, Jordan; Vasic, Tanja; Zornic, Vladimir; Racić, Nedeljko; Bekčić, Filip; Stepić, Marija; Kostić, Ivica
More profitable production and better forage quality is achieved by growing grasses and legumes in mixtures. Growing grasses in a mixture with legumes influences a significant amount of nitrogen fixed by Rhizobium bacteria and reduce the using of nitrogen mineral fertilization. Italian ryegrass is an ideal species for growing in a mixture with red clover. The aim of this study was to determine the nutritive value of Italian ryegrass – red clover mixtures depending on the seeding rate of these plants in the mixtures, in the fist and the second cut. Since the trial was not fertilized with N the legumes were required to supply N. The experiment was established at the experimental field of the Institute for forage crops Kruševac, Serbia, according to a total randomized block system in three replications. The results of this study showed that better nutritive value was determined in the mixtures with higher seeding rate of red clover in the mixture. In the first cut, the nutritive value of the mixture with the highest Italian ryegrass seeding rate was similar with nutritive value of red clover monocrop. Generally, in conclusion, growing Italian ryegrass in a bi-crop with red clover produces forage of high nutritive value suitable for ruminants feeding.
Penicillium crustosum - проузроковач плаве трулежи плодова нектарине
(Društvo za zaštitu bilja Srbije, 2022-11-28) Bekčić, Filip; Vico, I.; Vučković, N.; Mikić, S.; Stepić, Marija; Andjelković, Snežana; Duduk, Natasa
Primarily protein fractions by CNCPS (Cornell Net Carbohydrates and Protein System) of apple pomace silages
(Research Institute of Mountain Stockbreeding and Agriculture (RIMSA), 2022) Marković, Jordan; Lazarević, Đorđe; Racić, Nedeljko; Stepić, Marija; Bekčić, Filip; Kostić, I.; Vasić, Tanja
Apple pomace is a nutrient that can be included in a ruminant meal, as fresh, dried and ensiled. Drying the pomace would contribute to its inclusion in the concentrate mixture or as a single feed. The aim of this study was to quantify the primarily protein fractions by CNCPS (Cornell Net carbohydrate and Protein System) in apple pomace silages ensiled with dry beet pulp, sunflower meal and sugar beet pulp with inoculant andwithout inoculant. Apple pomace (AP) was obtained from the factory Vino Župa Aleksandrovac. Study treatments in the present research of the apple pomace sillage were: added feed (A) and applied inoculant (B): A1- ensiled apple pomace 100% participation (a1b1-apple pomace without inoculants; a1b2-apple pomace with inoculants; A2- apple pomace 85%+15% of dry beet pulp (a2b1-without inoculants; a2b2 with inoculants); A3-apple pomace 85%+15% sunflower meal (a3b1-without inoculants and a3b2 with inoculants); A4-apple pomace 84%+15% sugar beet pulp+Benural S 1% (a4b1-without inoculants and a4b2 with inoculants). Ensiled apple pomace with sunflower meal had the highest TP content, but the lowest NPN content of CP. Ensiled apple pomace with dry beet pulp had the highest IP, but the lowest SolP of CP. Treatments with inoculants had lower NDICP and ADICP than treatments without inoculants
Protein and carbohydrate profiles of a diploid and a tetraploid red clover cultivar
(Agricultural and Food Science, 2022-07-07) Marković, Jordan; Lazarević, Đorđe; Bekčić, Filip; Vasic, Tanja; Živković, Sanja; Štrbanović, Ratibor
Red clover (Trifolium pratense L.) is characterized by high dry matter (DM) yield and a high nutritive value. It has a higher concentration of soluble carbohydrate than lucerne and its protein is less degradable in the rumen than lucerne protein. The aim of this study was to quantify the main crude protein (CP) and carbohydrate (CHO) fractions of a tetraploid (4n, cv K-32) and a diploid (2n, cv K-39) red clover cultivar harvested at three stages of development in the spring growth. Results showed that the cultivar and stage of development had a large impact on chemical composition and the protein and carbohydrate fractions determined by the Cornell Net Carbohydrate and Protein System (CNCPS). The rapidly degradable protein fraction (PA) was significantly higher in the tetraploid (272 to 287 g kg-1 CP with advancing plant development) than in the diploid red clover cultivar (214 to 268 g kg-1 CP). Rumen degradable carbohydrate concentration was not affected by cultivar but it was significantly influenced by the stage of development. The concentration of rumen degradable protein decreased from 726 to 655 g kg-1 CP with advancing plant development in the diploid red clover cultivar. In conclusion, the tetraploid red clover cultivar was higher in CP, its protein was more rapidly degradable, and it was characterized by lower concentrations of lignin and unavailable carbohydrate fractions than the diploid cultivar.
Protein and carbohydrate profiles of a diploid and a tetraploid red clover cultivar
(Agricultural and Food Science, 2022-07-07) Marković, Jordan; Lazarević, Đorđe; Bekčić, Filip; Prijović, Mladen; Vasić; Živković; Štrbanović
Red clover (Trifolium pratense L.) is characterized by high dry matter (DM) yield and a high nutritive value. It has a higher concentration of soluble carbohydrate than lucerne and its protein is less degradable in the rumen than lucerne protein. The aim of this study was to quantify the main crude protein (CP) and carbohydrate (CHO) fractions of a tetraploid (4n, cv K-32) and a diploid (2n, cv K-39) red clover cultivar harvested at three stages of development in the spring growth. Results showed that the cultivar and stage of development had a large impact on chemical composition and the protein and carbohydrate fractions determined by the Cornell Net Carbohydrate and Protein System (CNCPS). The rapidly degradable protein fraction (PA) was significantly higher in the tetraploid (272 to 287 g kg-1 CP with advancing plant development) than in the diploid red clover cultivar (214 to 268 g kg-1 CP). Rumen degradable carbohydrate concentration was not affected by cultivar but it was significantly influenced by the stage of development. The concentration of rumen degradable protein decreased from 726 to 655 g kg-1 CP with advancing plant development in the diploid red clover cultivar. In conclusion, the tetraploid red clover cultivar was higher in CP, its protein was more rapidly degradable, and it was characterized by lower concentrations of lignin and unavailable carbohydrate fractions than the diploid cultivar.
Structural and non-structural carbohydrates content of apple pomace silages
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2022-10-06) Marković, Jordan; Vasić, Tanja; Stepić, Marija; Racić, Nedeljko; Bekčić, Filip; Lazarević, Đorđe; Kostić, Ivica
Apple pomace was ensiled without additives and with the addition of 15% dried beet pulp in order to increase the level of DM, 15% of sunflower meal and 15% dry beet pulp and 1% of NPN substances, and each of these treatments with and without inoculant. Apple pomace (AP) was obtained from the factory Vino Župa Aleksandrovac, delivered to the Institute of Forage Crops in Kruševac, location Globoder, on April 2nd 2013 and ensiling was performed on April 3rd 2013. Study treatments in the present research of the apple pomace silage were: added feed (A) and applied inoculant (B): A1 - ensiled apple pomace 100% participation (a1b1 - apple pomace without inoculants; a1b2 - apple pomace with inoculants; A2 - apple pomace 85% + 15% of dry beet pulp (a2b1 - without inoculants; a2b2 with inoculants); A3 - apple pomace 85% + 15% sunflower meal (a3b1 - without inoculants and a3b2 with inoculants); A4 - apple pomace 84% + 15% sugar beet pulp + Benural S 1% (a4b1 - without inoculants and a4b2 with inoculants). Results of these investigations showed that apple pomace silage with dry beet pulp had the highest CHO (817.8 g kg-1 DM), NFC (358.7 g kg-1 DM) and WSC (44.9 g kg-1 DM) contents. This study has shown that apple pomace silages, as a by-product of the technological process has significant nutritional value.
Suitability of field pea:oat and common vetch:oat mixtures for ensiling
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2021-10-07) Marković, Jordan; Vasić, T.; Petrović, Mirjana; Milenković, Jasmina; Bekčić, Filip; Lazarević, Đorđe; Babić, Snežana
The practice of preserving green crops by fermentation as silage has increased dramatically in recent years. Successful ensiling can be difficult due to numerous problems such as: high moisture content, high feed buffer capacity, insufficient amount of fermentable carbohydrates etc. The aim of this investigation was to determine the suitability for ensiling field pea:oat and common vetch:oat mixtures grown at different seeding rate and harvested at different stages of growth. Field pea, common vetch and oat were grown in pure stands and in the mixtures of 75:25, 50:50 and 25:75 ratios and harvested at three stages of plant development: beginning of field pea and common vetch flowering, forming the first pods in field pea and common vetch and at the forming green seeds in 2/3 field pea and common vetch pods. The most favorable ratio of sugar and buffer capacity for the established level of dry matter in the examined crops was stated in pure oat crop. The results obtained in the mixtures of field pea and oat are based on the ratioof sugar and buffer capacity and can successfully ensile in all three examined relations of germinating grains of field pea and oats. The highest sugar content (182.1 g kg-1 DM) and the highest buffer capacity were determined in the 50:50 field pea:oat mixture at the pea flowering stage of development. The least favorable ratio of sugar and buffer capacity was found in pure common vetch.
The effect of actinomycetes application on green mass yield of red clover
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2021-10-07) Andjelković, Snežana; Lugić, Zoran; Babić, Snežana; Milenković, Jasmina; Jevtić, Goran; Marković, Jordan; Bekčić, Filip
Red clover (Trifolium pratense L.) is one of the most important and widespread leguminous forage species in temperate agriculture, which is characterized by a high yield of quality biomass. Actinomycetes produce antibiotics and vitamins that act favourably on physiological processes in plants. These microorganisms are present in the soil, however the application of selected microorganisms for seed inoculation leads to better results in crop production. The experiment was carried out in vegetation pots in semi-controlled conditions. This research consisted of investigating the effect of actinomycetes (Streptomyces sp.) application on the green mass per plants of 12 cultivars of red clover of different geographical origin: K-27, K-32, K-38, K-39, Una, Kolubara, Viglana, Manuela, Wilo, Repio, Diana and Longevo. The effect of inoculation was determined out at the onset of flowering. The results obtained showed that the application of microorganisms could affect plant mass yield. The highest value for green mass per plant was achieved in cv. K-39 using inoculum that contained Streptomyces sp. The application of actinomycetes had a positive effect in the most cultivars of red clover and there was a statistically significant difference between inoculated treatments and control, indicating the justification of the use of these microorganisms in plant production.
The response different cultivars of red clover to microbial inoculation
(2022) Andjelković, Snežana; Lugić, Zoran; Babić, Snežana; Milenković, Jasmina; Jevtić, Goran; Bekčić, Filip; Petrović, Mirjana
This study aimed to evaluate the impact of microbial inoculationon on yield parameters different cultivars red clover (Trifolium pratense L.). This plant species is economical significance is based on high potential for production of biomass with high content of crude protein in biomass. It is a legume well adapted to poorly drained soils and tolerant to lower soil pH and fertility. The experiment was set up in a greenhouse, in the semi-controlled conditions. For the purposes of research ten commercial red clover cultivars of different geographical origin were used. The inoculation was done using Streptomyces sp. (actinomycetes).
The response of different cultivars birdsfoot trefoil (Lotus corniculatus L.) on pre-sowing inoculation
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2022-10-06) Andjelković, Snežana; Babić, Snežana; Milenković, Jasmina; Stepić, Marija; Marković, Jordan; Bekčić, Filip; Mitra, D.
Birdsfoot trefoil (Lotus corniculatus L.) is a widespread plant species, which is important in providing sufficient quantities of quality fodder on soils of low production potential. Like many other forage legumes, this plant species has a well developed symbiosis with rhizobia. The aim of this study was to evaluate the effect of pre-sowing inoculation on height and green mass of birdsfoot trefoil. The experiment was a two-factorial design, where the growth of plants influenced by three variants of microbial inoculation (individual cultures of Rhizobium loti, azotobacter (Azotobacter chroococcum) and actinomycetes (Streptomyces spp.)) was compared with the growth of noninoculated controls for three cultivars (K-37, Rocco and Bokor). For this study, plants were sown on acid soil with pH 4.91. Measurements were performed three times during two years of research: one cut in the first year and two cuts in the second year. For tested parameters, interaction between cultivar and inoculation resulted in a different effect compared to the control. Highest plant height was achieved in cv. Rocco: in the first year using actinomycetes and in two cuts of the second year using azotobacter. In this study minimum height was recorded in cv. Bokor. By using actinomycetes, the highest value for green mass was achieved in cv. K-37 in the first year and in the first cut of the second year. In this cultivar, using azotobacter the highest green mass in the second cut of the second year was achieved.
UTICAJ AZOTOBACTER SPP. NA POČETNI RAST I RAZVOJ VIŠEGODIŠNJIH TRAVA
(Institut za povrtarstvo Smederevska Palanka, 2023-11-02) Andjelković, Snežana; Sokolović, Dejan; Jevtić, Goran; Prijović, Mladen; Bekčić, Filip; Milenković, Jasmina; Babić, Snežana
Primena korisnih mikroorganizama, kao što su bakterije iz roda Azotobacter, predstavlja ekološki prihvatljiv metod za unapređenje proizvodnje krmnih trava i biogenosti zemljišta. U radu su prikazani rezultati primene predsetvene mikrobijalne inokulacije. Ispitivan je uticaj četiri izolata Azotobacter spp. (ZA1, SA74, SB94, MA7) i njihove kombinacije na početni rast višegodišnjih trava: engleskog ljulja (Lolium perenne L.), francuskog ljulja (Arrhenatherum elatius L.), italijanskog ljulja (Lolium multiflorum L.) i ježevice (Dactylis glomerata L.). Eksperiment je izveden u polukontrolisanim uslovima. Visina, zelena masa po biljci, dužina i masa korena po biljci određene su četiri nedelje nakon setve. Rezultati istraživanja pokazali su da je primena mikrobne inokulacije imala pozitivan efekat na početni rast i razvoj ispitivanih vrsta trava.
Wilt of red clover caused by Fusarium oxysporum
(Palacký University Olomouc, 2023-09-10) Bekčić, Filip; Stepić, Marija; Andjelković, Snežana; Marković, Jordan; Vučković, N; Vico, I; Duduk, Natasa
Red clover (Trifolium pratense L.) is an important forage legume in Serbia, grown in many regions, especially due to its ability to grow on acid soils. Red clover plants with symptoms of stunting, slight chlorosis and necrosis of leaves, discoloration and partial root rot were sampled in the field where red clover is grown in monoculture in Ćuprija, Serbia, in July, 2021. Several isolates were obtained from the roots and one (F16A) was further identified and characterized based on morphological, molecular and pathogenic characteristics.