Browsing by Author "Bekčić, Filip"

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ANTIFUNGAL ACTIVITY OF RHIZOBIA AGAINST RED CLOVER PATHOGENS FROM GENUS FUSARIUM
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia University of Belgrade, Faculty of Agriculture, Serbia Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB) Italy, 2023-10-05) Bekčić, Filip; Duduk, Natasa; Lugić, Zoran; Radović, Jasmina; Babić, Snežana; Stepić, Marija; Andjelković, Snežana
Red clover (Trifolium pratense L.) is an important forage legume that can be found in nature or as cultivated crop in monoculture or in grass-legume mixtures. The great importance of red clover is the fact that it lives in symbiosis with Rhizobium leguminosarum bv. trifoli. In addition to promoting plant growth, productivity and biomass, the bacteria also have an important role in disease control. Apart from useful microorganisms, many soil pathogenic microorganisms inhabit red clover roots and under favorable conditions they cause infections and diseases of the cultivated plants. On the infected red clover plants Fusarium spp. can cause several symptoms: vascular wilt on older plants, dumping of seedlings, root rot, rot of root base and crown rot. In the present study, the antagonistic potential was evaluated for two bacteria isolates preliminarily determined as R. leguminosarum bv. trifoli (CD1 and CD6) against three isolates Fusarium spp. (G1, G2 and G3) who were obtained from the roots of symptomatic red clover plants. The research was performed in in vitro agar plate (PDA medium) by methods of dual culture (“bacterial ring”). The comparison of growth assessment of fungi after they were grown together with rhizobia in a single plate and growth of fungi in individual plates was performed. Both rhizobia isolates showed significant antifungal activity against all the tested phytopathogenic fungi. Growth inhibition of G1, G2 and G3 was evaluated (%) using both rhizobia isolates, CD1 varied from 30 to 43 % and CD6 from 14 to 32 %.
ANTIFUNGALNA AKTIVNOST ETARSKOG ULJA SATUREJA MONTANA L. PREMA ALTERNARIA SP., PATOGENA STEPSKOG BOŽURA
(Društvo za zaštitu bilja Srbije, Nemanjina 6, 11080 Beograd, 2023-11-27) Mikić, Sara; Stević, Tatijana; Mrđan, Snežana; Vučković, Nina; Bekčić, Filip; Prijić, Željana; Marković, Tatijana
Rtanjski čaj, planinski čubar ili vrijesak (Satureja montana L.) je višegodišnja žbunasta biljka iz familije Lamiaceae, koja vodi poreklo iz umereno toplih područja Evrope, Mediterana i Afrike. U narodnoj medicini se koristi kao čaj za lečenje bolesti respiratornog, digestivnog i urinarnog sistema. Za spoljnu upotrebu se koristi prilikom upala kože i sluzokože, a kao začin i aromatični dodatak jelima u kulinarstvu. U industrijskoj preradi se koristi za dobijanje etarskog ulja. Etarsko ulje se dobija iz nadzemnog dela biljke. Hemijske komponente ulja su karvakrol, timol, β-kariofilen, γ-terpen, ρ-cimen, linalol i druge. U industriji parfema se kombinuje sa drugim uljima ili koristi samostalno. Cilj istraživanja bio je da se ispita antifungalna aktivnost tri hemotipa etarskog ulja Satureja montana L. prema Alternaria sp. izolovanu iz stabla stepskog božura (Paeonia tenuifolia L.).
GENETIČKI ODGOVOR LUCERKE NA TOKSIČNO DELOVANJE JONA ALUMINIJUMA NA KISELOJ HIDROPONSKOJ KULTURI
(Društvo genetičara Srbije, Beograd, 2023-10-16) Stepić, Marija; Bekčić, Filip; Prijović, Mladen; Radović, Jasmina; Zdravković, Jasmina
Toksičan efekat aluminijuma veoma je važan faktor koji ograničava rast i razviće korena lucerke (Medicago sativa L.) na kiselim zemljištima što dovodi do smanjenja prinosa. Cilj eksperimenta bio je da se utvrdi uticaj aluminijuma na: dužinu korena, svežu nadzemnu masu, svežu masu korena, broj bočnih korenova, dužinu bočnih korenova i na rastojanje do prvog bočnog grananja na korenu kod klijanaca tri sorte lucerke (Zuzana, K-28, O-66) u hidroponskoj kulturi u kontrolisanim uslovima. Korišćen je Hoaglandov rastvor ½ jačine pH vrednosti 4.5, za tretman je korišćen aluminijum u formi aluminijum-hlorida (koncentracije 100 µM) i za analize je korišćeno 40 klijanaca po sorti.
NUTRITIVE VALUE OF ITALIAN RYEGRASS-RED CLOVER MIXTURES DEPENDING ON THE SEEDING RATE IN THE MIXTURES
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia University of Belgrade, Faculty of Agriculture, Serbia Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB) Italy, 2023-10-05) Marković, Jordan; Vasic, Tanja; Zornic, Vladimir; Racić, Nedeljko; Bekčić, Filip; Stepić, Marija; Kostić, Ivica
More profitable production and better forage quality is achieved by growing grasses and legumes in mixtures. Growing grasses in a mixture with legumes influences a significant amount of nitrogen fixed by Rhizobium bacteria and reduce the using of nitrogen mineral fertilization. Italian ryegrass is an ideal species for growing in a mixture with red clover. The aim of this study was to determine the nutritive value of Italian ryegrass – red clover mixtures depending on the seeding rate of these plants in the mixtures, in the fist and the second cut. Since the trial was not fertilized with N the legumes were required to supply N. The experiment was established at the experimental field of the Institute for forage crops Kruševac, Serbia, according to a total randomized block system in three replications. The results of this study showed that better nutritive value was determined in the mixtures with higher seeding rate of red clover in the mixture. In the first cut, the nutritive value of the mixture with the highest Italian ryegrass seeding rate was similar with nutritive value of red clover monocrop. Generally, in conclusion, growing Italian ryegrass in a bi-crop with red clover produces forage of high nutritive value suitable for ruminants feeding.
Primarily protein fractions by CNCPS (Cornell Net Carbohydrates and Protein System) of apple pomace silages
(Research Institute of Mountain Stockbreeding and Agriculture (RIMSA), 2022) Marković, Jordan; Lazarević, Đorđe; Racić, Nedeljko; Stepić, Marija; Bekčić, Filip; Kostić, I.; Vasić, Tanja
Apple pomace is a nutrient that can be included in a ruminant meal, as fresh, dried and ensiled. Drying the pomace would contribute to its inclusion in the concentrate mixture or as a single feed. The aim of this study was to quantify the primarily protein fractions by CNCPS (Cornell Net carbohydrate and Protein System) in apple pomace silages ensiled with dry beet pulp, sunflower meal and sugar beet pulp with inoculant andwithout inoculant. Apple pomace (AP) was obtained from the factory Vino Župa Aleksandrovac. Study treatments in the present research of the apple pomace sillage were: added feed (A) and applied inoculant (B): A1- ensiled apple pomace 100% participation (a1b1-apple pomace without inoculants; a1b2-apple pomace with inoculants; A2- apple pomace 85%+15% of dry beet pulp (a2b1-without inoculants; a2b2 with inoculants); A3-apple pomace 85%+15% sunflower meal (a3b1-without inoculants and a3b2 with inoculants); A4-apple pomace 84%+15% sugar beet pulp+Benural S 1% (a4b1-without inoculants and a4b2 with inoculants). Ensiled apple pomace with sunflower meal had the highest TP content, but the lowest NPN content of CP. Ensiled apple pomace with dry beet pulp had the highest IP, but the lowest SolP of CP. Treatments with inoculants had lower NDICP and ADICP than treatments without inoculants
Protein and carbohydrate profiles of a diploid and a tetraploid red clover cultivar
(Agricultural and Food Science, 2022-07-07) Marković, Jordan; Lazarević, Đorđe; Bekčić, Filip; Vasic, Tanja; Živković, Sanja; Štrbanović, Ratibor
Red clover (Trifolium pratense L.) is characterized by high dry matter (DM) yield and a high nutritive value. It has a higher concentration of soluble carbohydrate than lucerne and its protein is less degradable in the rumen than lucerne protein. The aim of this study was to quantify the main crude protein (CP) and carbohydrate (CHO) fractions of a tetraploid (4n, cv K-32) and a diploid (2n, cv K-39) red clover cultivar harvested at three stages of development in the spring growth. Results showed that the cultivar and stage of development had a large impact on chemical composition and the protein and carbohydrate fractions determined by the Cornell Net Carbohydrate and Protein System (CNCPS). The rapidly degradable protein fraction (PA) was significantly higher in the tetraploid (272 to 287 g kg-1 CP with advancing plant development) than in the diploid red clover cultivar (214 to 268 g kg-1 CP). Rumen degradable carbohydrate concentration was not affected by cultivar but it was significantly influenced by the stage of development. The concentration of rumen degradable protein decreased from 726 to 655 g kg-1 CP with advancing plant development in the diploid red clover cultivar. In conclusion, the tetraploid red clover cultivar was higher in CP, its protein was more rapidly degradable, and it was characterized by lower concentrations of lignin and unavailable carbohydrate fractions than the diploid cultivar.
Protein and carbohydrate profiles of a diploid and a tetraploid red clover cultivar
(Agricultural and Food Science, 2022-07-07) Marković, Jordan; Lazarević, Đorđe; Bekčić, Filip; Prijović, Mladen; Vasić; Živković; Štrbanović
Red clover (Trifolium pratense L.) is characterized by high dry matter (DM) yield and a high nutritive value. It has a higher concentration of soluble carbohydrate than lucerne and its protein is less degradable in the rumen than lucerne protein. The aim of this study was to quantify the main crude protein (CP) and carbohydrate (CHO) fractions of a tetraploid (4n, cv K-32) and a diploid (2n, cv K-39) red clover cultivar harvested at three stages of development in the spring growth. Results showed that the cultivar and stage of development had a large impact on chemical composition and the protein and carbohydrate fractions determined by the Cornell Net Carbohydrate and Protein System (CNCPS). The rapidly degradable protein fraction (PA) was significantly higher in the tetraploid (272 to 287 g kg-1 CP with advancing plant development) than in the diploid red clover cultivar (214 to 268 g kg-1 CP). Rumen degradable carbohydrate concentration was not affected by cultivar but it was significantly influenced by the stage of development. The concentration of rumen degradable protein decreased from 726 to 655 g kg-1 CP with advancing plant development in the diploid red clover cultivar. In conclusion, the tetraploid red clover cultivar was higher in CP, its protein was more rapidly degradable, and it was characterized by lower concentrations of lignin and unavailable carbohydrate fractions than the diploid cultivar.
Structural and non-structural carbohydrates content of apple pomace silages
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2022-10-06) Marković, Jordan; Vasić, Tanja; Stepić, Marija; Racić, Nedeljko; Bekčić, Filip; Lazarević, Đorđe; Kostić, Ivica
Apple pomace was ensiled without additives and with the addition of 15% dried beet pulp in order to increase the level of DM, 15% of sunflower meal and 15% dry beet pulp and 1% of NPN substances, and each of these treatments with and without inoculant. Apple pomace (AP) was obtained from the factory Vino Župa Aleksandrovac, delivered to the Institute of Forage Crops in Kruševac, location Globoder, on April 2nd 2013 and ensiling was performed on April 3rd 2013. Study treatments in the present research of the apple pomace silage were: added feed (A) and applied inoculant (B): A1 - ensiled apple pomace 100% participation (a1b1 - apple pomace without inoculants; a1b2 - apple pomace with inoculants; A2 - apple pomace 85% + 15% of dry beet pulp (a2b1 - without inoculants; a2b2 with inoculants); A3 - apple pomace 85% + 15% sunflower meal (a3b1 - without inoculants and a3b2 with inoculants); A4 - apple pomace 84% + 15% sugar beet pulp + Benural S 1% (a4b1 - without inoculants and a4b2 with inoculants). Results of these investigations showed that apple pomace silage with dry beet pulp had the highest CHO (817.8 g kg-1 DM), NFC (358.7 g kg-1 DM) and WSC (44.9 g kg-1 DM) contents. This study has shown that apple pomace silages, as a by-product of the technological process has significant nutritional value.
The response of different cultivars birdsfoot trefoil (Lotus corniculatus L.) on pre-sowing inoculation
(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2022-10-06) Andjelković, Snežana; Babić, Snežana; Milenković, Jasmina; Stepić, Marija; Marković, Jordan; Bekčić, Filip; Mitra, D.
Birdsfoot trefoil (Lotus corniculatus L.) is a widespread plant species, which is important in providing sufficient quantities of quality fodder on soils of low production potential. Like many other forage legumes, this plant species has a well developed symbiosis with rhizobia. The aim of this study was to evaluate the effect of pre-sowing inoculation on height and green mass of birdsfoot trefoil. The experiment was a two-factorial design, where the growth of plants influenced by three variants of microbial inoculation (individual cultures of Rhizobium loti, azotobacter (Azotobacter chroococcum) and actinomycetes (Streptomyces spp.)) was compared with the growth of noninoculated controls for three cultivars (K-37, Rocco and Bokor). For this study, plants were sown on acid soil with pH 4.91. Measurements were performed three times during two years of research: one cut in the first year and two cuts in the second year. For tested parameters, interaction between cultivar and inoculation resulted in a different effect compared to the control. Highest plant height was achieved in cv. Rocco: in the first year using actinomycetes and in two cuts of the second year using azotobacter. In this study minimum height was recorded in cv. Bokor. By using actinomycetes, the highest value for green mass was achieved in cv. K-37 in the first year and in the first cut of the second year. In this cultivar, using azotobacter the highest green mass in the second cut of the second year was achieved.
UTICAJ AZOTOBACTER SPP. NA POČETNI RAST I RAZVOJ VIŠEGODIŠNJIH TRAVA
(Institut za povrtarstvo Smederevska Palanka, 2023-11-02) Andjelković, Snežana; Sokolović, Dejan; Jevtić, Goran; Prijović, Mladen; Bekčić, Filip; Milenković, Jasmina; Babić, Snežana
Primena korisnih mikroorganizama, kao što su bakterije iz roda Azotobacter, predstavlja ekološki prihvatljiv metod za unapređenje proizvodnje krmnih trava i biogenosti zemljišta. U radu su prikazani rezultati primene predsetvene mikrobijalne inokulacije. Ispitivan je uticaj četiri izolata Azotobacter spp. (ZA1, SA74, SB94, MA7) i njihove kombinacije na početni rast višegodišnjih trava: engleskog ljulja (Lolium perenne L.), francuskog ljulja (Arrhenatherum elatius L.), italijanskog ljulja (Lolium multiflorum L.) i ježevice (Dactylis glomerata L.). Eksperiment je izveden u polukontrolisanim uslovima. Visina, zelena masa po biljci, dužina i masa korena po biljci određene su četiri nedelje nakon setve. Rezultati istraživanja pokazali su da je primena mikrobne inokulacije imala pozitivan efekat na početni rast i razvoj ispitivanih vrsta trava.
Wilt of red clover caused by Fusarium oxysporum
(Palacký University Olomouc, 2023-09-10) Bekčić, Filip; Stepić, Marija; Andjelković, Snežana; Marković, Jordan; Vučković, N; Vico, I; Duduk, Natasa
Red clover (Trifolium pratense L.) is an important forage legume in Serbia, grown in many regions, especially due to its ability to grow on acid soils. Red clover plants with symptoms of stunting, slight chlorosis and necrosis of leaves, discoloration and partial root rot were sampled in the field where red clover is grown in monoculture in Ćuprija, Serbia, in July, 2021. Several isolates were obtained from the roots and one (F16A) was further identified and characterized based on morphological, molecular and pathogenic characteristics.