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Item Primarily protein fractions by CNCPS (Cornell Net Carbohydrates and Protein System) of apple pomace silages(Research Institute of Mountain Stockbreeding and Agriculture (RIMSA), 2022) Marković, Jordan; Lazarević, Đorđe; Racić, Nedeljko; Stepić, Marija; Bekčić, Filip; Kostić, I.; Vasić, TanjaApple pomace is a nutrient that can be included in a ruminant meal, as fresh, dried and ensiled. Drying the pomace would contribute to its inclusion in the concentrate mixture or as a single feed. The aim of this study was to quantify the primarily protein fractions by CNCPS (Cornell Net carbohydrate and Protein System) in apple pomace silages ensiled with dry beet pulp, sunflower meal and sugar beet pulp with inoculant andwithout inoculant. Apple pomace (AP) was obtained from the factory Vino Župa Aleksandrovac. Study treatments in the present research of the apple pomace sillage were: added feed (A) and applied inoculant (B): A1- ensiled apple pomace 100% participation (a1b1-apple pomace without inoculants; a1b2-apple pomace with inoculants; A2- apple pomace 85%+15% of dry beet pulp (a2b1-without inoculants; a2b2 with inoculants); A3-apple pomace 85%+15% sunflower meal (a3b1-without inoculants and a3b2 with inoculants); A4-apple pomace 84%+15% sugar beet pulp+Benural S 1% (a4b1-without inoculants and a4b2 with inoculants). Ensiled apple pomace with sunflower meal had the highest TP content, but the lowest NPN content of CP. Ensiled apple pomace with dry beet pulp had the highest IP, but the lowest SolP of CP. Treatments with inoculants had lower NDICP and ADICP than treatments without inoculantsItem Inhibitory effect of an emulsion of Nepeta rtanjensis essential oil on Amaranthus retroflexus seed germination(Serbian Plant Physiology Society, 2022-10-06) Jovanović, V.; Prijović, Mladen; Nikolić, B.; Dragićević, I.; Nestorović Živković, J.; Dmitrović, S.; Giba, Z.Item Botanical composition and biomass quality in natural grasslands of southeast part of Serbia(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia University of Belgrade, Faculty of Agriculture, Serbia Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB) Italy, 2022-10-06) Zornic, Vladimir; Petrović, Mirjana; Babić, Snežana; Sokolović, Dejan; Prijović, Mladen; Tomić, Dalibor; Lazarević, ĐorđeItem Influence of stimulative feeding measures on spring development of bee societies in DB and LR honeycombs(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2022-10-06) Jevtić, Goran; Babić, Snežana; Andjelković, Snežana; Zornic, Vladimir; Lazarević, Đorđe; Matovic, Kazimir; Nedić, N.In order for the bee colonies to make the most of the main pasture, and primarily black locust, it is very important that they are in good condition. It is necessary for the societies to reach their maximum strength at the beginning of the main pasture, and not for it to serve them for further development. The beekeeper himself plays a crucial role in the accelerated spring development of bee colonies. The aim of this study was to determine the effect of different ways of spring feeding on the strength of bee colonies and the food supply in them. The experiment was performed with companies that were in Db (Dadant-Blatt) and Lr (Langstroth-Ruth) hives. Four groups of companies were tested, and there were five companies in each group. The first group was supplemented with sugar syrup, enriched with vitamin-mineral complex - Foprsapine (10 drops per liter of syrup). The second group of societies was fed with honey dissolved in water (ratio of honey and water 1:1). The third group of the companies was fed with sugar dough (energy cake without additives). The fourth group of companies was supplemented with sugar syrup (the ratio of water and sugar was 1:1). At the beginning of the experiment, the societies in Lr hives had an average of 4.5 frames with bees, and 2.5 frames with a brood. In the Db hives, the societies occupied five frames with bees, and had 2.7 frames with a brood. At the end of the experiment Lr societies had 8.7 frames with bees, and 4.5 frames with brood, while Db societies had nine frames with bees, and 4.7 frames with brood. The experiment was performed at the apiary of the Institute for Forage Crops in Kruševac.Item Forage quality of different Festulolium cultivars(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2022-10-06) Babić, Snežana; Sokolović, Dejan; Andjelković, Snežana; Petrović, Mirjana; Jevtić, Goran; Prijović, Mladen; Racić, NedeljkoHybrids created by crossing between genera Festuca and Lolium species are Festulolium.Crossing species of these genera aim to combine their positive characteristics, such as Festuca tolerance to abiotic stresses and edaphic and climatic conditions with the high quality and digestibility of Lolium perenne and Lolium multiflorum species. Forage quality of 15 different Lolioid Festulolium cultivars and six cultivars of Festuca and Lolium pure species were investigated in a moderate continental climate in Kruševac, Serbia. Plots in the trial (7,5 m2 ) were completely randomized, each in three replications. In the year of establishment, the plots were cut without weighing and taking samples. In the next two years, two cuts were taken. The first cut was done at the beginning of the heading (the first half of May) and the second in the first half of July. On dry samples, from two cuts, the content of crude protein, crude cellulose, crude fat, ash, ADF, NDF and ADL was determined by standard laboratory methods. Results were presented as two-year average values. The best dry matter quality of Festulolium cultivars was determined for cultivars AberNiche and Felopa. The highest crude protein content in the first cut was determined for AberNiche (187.1 gkg-1 ) and Felopa (169.6 gkg-1). Also, the same cultivars noted the lowest values for ADF (296.7 and 303.5 gkg-1 ) and for AberNiche was determined the lowest ADL contet (35.4 gkg-1 ) in the first cut. These cultivars are in the group of cultivars with the lowest NDF value. Based on the obtained results, it can be concluded that the analyzed Festulolium cultivars have a higher crude protein content compared to the cultivars of Festuca pure species, but lower than Lolium species.Item Structural and non-structural carbohydrates content of apple pomace silages(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2022-10-06) Marković, Jordan; Vasić, Tanja; Stepić, Marija; Racić, Nedeljko; Bekčić, Filip; Lazarević, Đorđe; Kostić, IvicaApple pomace was ensiled without additives and with the addition of 15% dried beet pulp in order to increase the level of DM, 15% of sunflower meal and 15% dry beet pulp and 1% of NPN substances, and each of these treatments with and without inoculant. Apple pomace (AP) was obtained from the factory Vino Župa Aleksandrovac, delivered to the Institute of Forage Crops in Kruševac, location Globoder, on April 2nd 2013 and ensiling was performed on April 3rd 2013. Study treatments in the present research of the apple pomace silage were: added feed (A) and applied inoculant (B): A1 - ensiled apple pomace 100% participation (a1b1 - apple pomace without inoculants; a1b2 - apple pomace with inoculants; A2 - apple pomace 85% + 15% of dry beet pulp (a2b1 - without inoculants; a2b2 with inoculants); A3 - apple pomace 85% + 15% sunflower meal (a3b1 - without inoculants and a3b2 with inoculants); A4 - apple pomace 84% + 15% sugar beet pulp + Benural S 1% (a4b1 - without inoculants and a4b2 with inoculants). Results of these investigations showed that apple pomace silage with dry beet pulp had the highest CHO (817.8 g kg-1 DM), NFC (358.7 g kg-1 DM) and WSC (44.9 g kg-1 DM) contents. This study has shown that apple pomace silages, as a by-product of the technological process has significant nutritional value.Item Heredity mode of duration of vegetative stage in onion (Allium cepa L.)(University of Banja Luka Faculty of Agriculture, 2022-05-26) Pavlović, Nenad; Marjanović, Miloš; Mladenovic, Jelena; Tomić, Dalibor; Bošković-Rakočević, Ljiljana; Moravcevic, Djordje; Zdravković, JasminaThe duration of the vegetation period is a very important biological property of onion. In order to determine the ways of inheriting the duration of the vegetation period, the method of full diallel without reciprocals was applied, in order to obtain offspring of F1 and F2 generation. The field experiment with parents and hybrids of F1 and F2 generation was set up according to a random block system with five repetitions at the Institute for Vegetable Crops, Smederevska Palanka. The mode of inheritance of early maturity in this experiment was superdominance, observing all crossing combinations. The best general combiner in both generations was the AC 101 line. The highest significant value for SCA in both the F1 and F2 generations had the hybrid created by crossing Piroška x AC 101.Item The response of different cultivars birdsfoot trefoil (Lotus corniculatus L.) on pre-sowing inoculation(University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2022-10-06) Andjelković, Snežana; Babić, Snežana; Milenković, Jasmina; Stepić, Marija; Marković, Jordan; Bekčić, Filip; Mitra, D.Birdsfoot trefoil (Lotus corniculatus L.) is a widespread plant species, which is important in providing sufficient quantities of quality fodder on soils of low production potential. Like many other forage legumes, this plant species has a well developed symbiosis with rhizobia. The aim of this study was to evaluate the effect of pre-sowing inoculation on height and green mass of birdsfoot trefoil. The experiment was a two-factorial design, where the growth of plants influenced by three variants of microbial inoculation (individual cultures of Rhizobium loti, azotobacter (Azotobacter chroococcum) and actinomycetes (Streptomyces spp.)) was compared with the growth of noninoculated controls for three cultivars (K-37, Rocco and Bokor). For this study, plants were sown on acid soil with pH 4.91. Measurements were performed three times during two years of research: one cut in the first year and two cuts in the second year. For tested parameters, interaction between cultivar and inoculation resulted in a different effect compared to the control. Highest plant height was achieved in cv. Rocco: in the first year using actinomycetes and in two cuts of the second year using azotobacter. In this study minimum height was recorded in cv. Bokor. By using actinomycetes, the highest value for green mass was achieved in cv. K-37 in the first year and in the first cut of the second year. In this cultivar, using azotobacter the highest green mass in the second cut of the second year was achieved.Item The importance of fodder legumes in the provision of animal feed(The Balkans Scientific Center of the Russian Academy of Natural Sciences, 2022-06-29) Stevović, Vladeta; Djukić, D.; Tomić, Dalibor; Djurović, D.; Lazarević, Đorđe; Madic, Milomirka; Marjanović, Miloš; Pavlovic, N.Item Interdependence of seed yield components of pumpkin (Cucurbita pepo L.) genotypes(University of Banja Luka Faculty of Agriculture, 2022-05-26) Tomić, Dalibor; Marjanović, Miloš; Radovanovic, M.; Djurović, V.; Lazarević, Đorđe; Stevović, Vladeta; Pavlovic, N.The aim of this study was to analyze the interdependence of seed yield components of the pumpkin (Cucurbita pepo L.) genotypes, which are grown in Western and Central Serbia. The experiment was performed in the period 2021-2022. In the initial phase, the fruits of genotypes of pumpkin grown in the villages from Priboj in the south to Šabac in the north, Smederevska Palanka and Krusevac in the east were collected. A total of 81 pumpkin fruits of 27 different genotypes were gathered. The following yield components were analyzed on selected fruits: fruit weight, number of seeds per fruit, dry seed weight per fruit, thousand seed weight, kernel weight per fruit and kernel percentage. Fruit weight was significantly positively correlated with number of seeds per fruit, dry seed weight per fruit, kernel weight per fruit and thousand seed weight, and significantly negatively correlated with kernel percentage (r = -0.44). Fruit weight was not significantly correlated with number of seeds per fruit, while number of seeds per fruit was significantly positively correlated with seeds weight per fruit and kernel weight per fruit. Seeds weight per fruit was in a significant positive correlation with kernel weight per fruit and thousand seed weight. Kernel percentage was significantly positively correlated with the number of seeds per fruit, seeds weight per fruit, kernel weight per fruit and the thousand seed weight. The analyzed genotypes represent an important source of diversity that could be used in plant breeding studies in the future.Item Assessment of quality and chemical composition of continental halophytic grasslands in south-east Europe(University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 2022-03-21) LUKOVIĆ; ŠILC; VASIN; Radović, Jasmina; TOPISIROVIĆ; KOSTIĆ; DAJIĆ STEVANOVIĆContinental halophytic grasslands are known for performing of range of ecosystem services especially remarkable in the regions where they are much distributed – in arid and semi-arid areas. Continental halophytic grasslands of the Central and South-East Europe are not considered as favourable for arable farming, however, traditional animal husbandry plays a crucial role in maintaining biodiversity and preserving these natural habitats. The particular interest of this study is the assessment of the chemical composition of biomass and the quality of insufficiently studied halophytic grassland communities of the central Balkans. In addition, the differences in pastoral value were monitored along the geographical gradient, i.e., between grasslands situated in the Pannonian plain (grasslands of Alliance Puccinellion limosae) and those distributed on the south Serbia (Alliance Festucion pseudovinae). The study was carried out at 18 representative sites, focusing on the saline grasslands of the two distinct regions – on the north and on the south of Serbia. The obtained results show that the halophytic grasslands have satisfying quality characteristics (average values: dry matter - 93.67%, crude protein - 8.66%, cellulose - 30.36%, crude fat - 2.27%) compared with other studied grasslands of saline habitats. There were no significant differences in quality and chemical composition of grasslands of the two regions, despite differences in floristic composition, indicating that salinity is the key determinant for pastoral value of the halophytic vegetation. The certain variations were attributed to the specific floristic composition related to ecological conditions and halophytic community characteristics.Item The change of phytochemical profile in beet juice and the influence of different storage conditions during one year(University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 2022-09-23) PAVLOVIĆ; ZDRAVKOVIĆ; MLADENOVIĆ; TOMIĆ; MARJANOVIĆ; MORAVČEVIĆ; Zdravković, JasminaMany scientific researches proved the antioxidative impact of beet and its products. The use of vegetable juices is getting more popular in human diet. The way of storage plays an important role in preservation, long life and minimal variability of phytonutrients. The storage of products, usually in inappropriate conditions, leads to additional loss of phytonutrients, which have already been decreased by processing. In this research, we studied the impact of three ways of storage of pasteurised beet juice during one year, on content of some nutrients (total sugars, vitamin C, phenols and total antioxidative capacity). Pasteurised juice was stored in three ways: in light, at room temperature, in dark, at room temperature and in dark, at temperature of 4 °C. The change of content and differences have been followed during one month and confirmed with ANOVA and Tukey's test. The lowest changes of total sugars have been recorded in storage in dark at 4 °C, while in storage in light, the sugar content increased. The losses of vitamin C during one year of storage had linear trend of decrease. Antioxidative capacity of beet juice depends on concentration of phenol compounds and loss of these parameters was similar during period of one year. The best way of storage was dark place at low temperature.Item ENVIRONMENTAL ASSESSMENT OF SOME HEAVY METALS IN SELECTED MEDICINAL PLANT SPECIES ALONG A BUSY ROAD IN VRNJAČKA BANJA, SERBIA(Asociatia Carpatica de Mediu si Stiintele Pamantului, 2022-08-31) BABIĆ; MITRA; DAS MOHAPATRA; PAPOVIĆ; Milenković, Jasmina; Jevtić, Goran; Babić, Snežana; Andjelković, SnežanaThis study reported the concentrations of Pb, Cd, Cr, Mn in parts of four medicinal plant species (Cichorium intybus L., Mentha×piperita L., Plantago lanceolata L., Verbascum densiflorum Bertol.) collected from two sites, one few meters away and the other 500 m away from a busy road in Vrnjačka Banja, Serbia. Heavy metals were determined by the atomic absorption method using atomic absorption spectrophotometer. Similar content of tested metals in the soil of the tested locations was recorded, only there was more manganese in the soil closer to the road. The highest concentrations of heavy metals were found in the roots of plants closer to the road. The leaves and stem contain smaller quantities of these metals than root, but there are differences between the studied plant species. In conclusion, all examined metals were present in higher concentrations in plants at the location closest to the road than at the location further away from the road.Item Fertility and chemical composition of forest soils covered with Allium ursinum L. In Serbia(2022-04) Dželetović, S. Ž.; Simić, A.; Marković, Jordan; Andrejić, G.; Denader, T.; Babić, SnežanaItem Fertility and chemical composition of forest soils covered with Allium ursinum L. In Serbia(Parlar Scientific Publications, 2022-04) Dželetović, Ž.,; Simić, A.; Marković, Jordan; Andrejić, G.; Denader, T.; Babić, SnežanaA. ursinum is a wild growing species, which is often found on various localities throughout Serbia, primarily within forest and occasionally on meadow. Fresh picked leaves and bulbs are used for preparing a salad or meals. For this research we have collected and analyzed soil and plant material from 12 different locations in Serbia. The analyses of basic fertility of soils have shown that A. ursinum grows mainly on acidic and soils containing high percentage of humus, with high total nitrogen content, low to medium supplies in available phosphorus and high supplies in available potassium. In those soils there is a very strong correlation between the total N content and total organic C (0.931). There is a relatively wide range of concentrations of overall Cd, Co, Cr, Cu, Ni, Pb and Zn in soils on which A. ursinum grows. The translocation factor for A. ursinum is TF ≥1 has been calculated for Cd, Pb and Zn.Item The effect of supplementing chestnut tannins on the productive results of finishing lambs fed rations deficient in metabolizable protein(2022) Cekić, B.; Božičković, A.; Ružić-Muslić, D.; Marković, Jordan; Maksimović, N.; Stanisavljević, N.Item Protein fractions as influenced by cultivars, stage of maturity and cutting dates in alfalfa (Medicago sativa L.)(Agricultural Research Communication Center, 2019-05-08) Marković, Jordan; Petrović, Mirjana; Terzić, Dragan; Vasić, Tanja; Kostić, Ivica; Štrbanović, Ratibor; Grubić, G.This study was undertaken to determine the relationship between CNCPS (Cornell Net Carbohydrate and Protein System) protein fractions and in vitro RUP (Rumen Undegradable Protein) concentration and the variability of protein fractions among alfalfa cultivars grown in Serbia. Two cultivars of alfalfa (Medicago sativa L.) – Serbian cv K 28 and American cv G + 13R + CZ were sampled at three stages of maturity. Comparing the two cultivars of alfalfa (K 28 vs. G + 13R + CZ) means, there were significant differences in all protein fractions. Two investigated alfalfa cultivars differed significantly (p less than 0.01) in RUP content, cv G + 13R + CZ was higher in RUP than cv K 28. Stage of maturity had an effect on proportions of the protein fractions. From a nutritional and breeding point of view, cultivar such as G + 13R + CZ is desirable because it combine higher CP (Crude Protein) values with lower protein degradability than cv K 28.Item Morphological and Chemical Characterization of a Collection of Mountain Clover Natural Populations(Institutul Național de Cercetare Dezvoltare Agricolă Fundulea, 2022) Petrović, Mirjana; Lugić, Zoran; Zornic, Vladimir; Andjelković, Snežana; Tomić, Dalibor; Milenković, Jasmina; Jevtić, GoranWith climate change evident, the possibility opens up of introducing into production a species that, although not characterized by high yield, nevertheless offers some other benefits for both the environment and man. One of these species is mountain clover (Trifolium montanum L.), a species widespread around European in the past, but due to agricultural activities its habitat has become fragmented and areas of mountain clover impaired. In the present study, the collection of nine natural populations of mountain clover originating from different parts of the hilly-mountainous areas of Serbia was tested in field conditions. We analysed different morphological traits (green plant biomass, stem length, internode number, number of lateral branches, leaf length and leaf width), dry matter quality traits (content of crude proteins, crude fibre and crude fat) as well as secondary metabolites (total phenolic content, flavonoid contents and antioxidative activity). We collected morphological data and plant samples during 2011 and 2012. We performed descriptive statistics to provide basic information about variables in the dataset, then calculated Shannon-Weaver diversity index (H’) and performed two-way ANOVA and principal component analyses (PCA). Analysing the broad range of data collected during two years, we found considerable morphological and chemical diversity amongst the collection of mountain clovers from central Serbia. Mean coefficient of variation (CV) in the morphological dataset ranged from 18% (stem length) to 57.6% (plant biomass) in 2011 and from 16.5% (leaf length) to 70.6% (stem number) in 2012. Dry matter (DM) parameters displayed the lowest CV, ranging from 6.1% (crude proteins) to 14.8% (crude fat), indicating that these parameters were less discriminative within the study collection. Over all populations, average crude protein content was 19.5%, and average crude fibre content was 27.3%. Total phenolic contents (expressed as gallic acid equivalent, GAE) ranged from 49.8 to 89.7 mg GAE g-1 DM, and flavonoid contents (expressed as rutin, Ru, equivalent) ranged from 66.8 to 142 mg Ru g-1 DM. Average antioxidative activity expressed in terms of IC50 values ranged from 177 to 426 mg ml-1 of methanol extract.Item Protein and carbohydrate profiles of a diploid and a tetraploid red clover cultivar(Agricultural and Food Science, 2022-07-07) Marković, Jordan; Lazarević, Đorđe; Bekčić, Filip; Prijović, Mladen; Vasić; Živković; ŠtrbanovićRed clover (Trifolium pratense L.) is characterized by high dry matter (DM) yield and a high nutritive value. It has a higher concentration of soluble carbohydrate than lucerne and its protein is less degradable in the rumen than lucerne protein. The aim of this study was to quantify the main crude protein (CP) and carbohydrate (CHO) fractions of a tetraploid (4n, cv K-32) and a diploid (2n, cv K-39) red clover cultivar harvested at three stages of development in the spring growth. Results showed that the cultivar and stage of development had a large impact on chemical composition and the protein and carbohydrate fractions determined by the Cornell Net Carbohydrate and Protein System (CNCPS). The rapidly degradable protein fraction (PA) was significantly higher in the tetraploid (272 to 287 g kg-1 CP with advancing plant development) than in the diploid red clover cultivar (214 to 268 g kg-1 CP). Rumen degradable carbohydrate concentration was not affected by cultivar but it was significantly influenced by the stage of development. The concentration of rumen degradable protein decreased from 726 to 655 g kg-1 CP with advancing plant development in the diploid red clover cultivar. In conclusion, the tetraploid red clover cultivar was higher in CP, its protein was more rapidly degradable, and it was characterized by lower concentrations of lignin and unavailable carbohydrate fractions than the diploid cultivar.Item Actinobacteria-enhanced plant growth, nutrient acquisition, and crop protection: Advances in soil, plant, and microbial multifactorial interactions(Elsevier BV, 2022-02) MITRA; MONDAL; KHOSHRU; SENAPATI; RADHA; MAHAKUR; UNIYAL; MYO; BOUTAJ; SIERRA; PANNEERSELVAM; GANESHAMURTHY; Andjelković, Snežana; VASIĆ; RANI; DUTTA; MOHAPATRA